Fig. 3 | Orphanet Journal of Rare Diseases

Fig. 3

From: Protein signature of human skin fibroblasts allows the study of the molecular etiology of rare neurological diseases

Fig. 3

Single cell histogram analysis of BODIPY stained skin fibroblasts: a control sample and b AAAS mutant sample (Allgrove-patient), c evaluation of fluorescence intensity. Two control samples and two replicates of the AAAS mutant sample were analyzed. Hereby, at least 30 individual cells per sample were chosen for statistical analysis. For each cell, a histogram analysis of intensity values was performed, results were normalized to percentage values and the percentage values of all intensities exceeding 50% of Imax were summed up. The resulting data are depicted as box plot diagrams. Cells from AAAS samples have significantly more pixels of high intensity than control samples (p < 0.001)

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