Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: STED lithography in microfluidics for 3D thrombocyte aggregation testing

Fig. 2

Estimation of the number of vWF molecules on nanoanchors. a Examples of fluorescence peaks on nanoanchors carrying most probably (from top to bottom) one (809 counts), two (1527 counts), three (2563 counts), and four (3306 counts) fluorescent IgG antibodies. b Distribution of Alexa647 fluorescence signals (a.u.) from anti-vWF IgGs. Purple: single antibody signals originating from sparsely and randomly distributed antibodies on a piranha-cleaned glass surface; green: fluorescence signals from nanoanchors loaded with vWF. Illumination time: 5 ms. c Black line: Probability density function fitted to the experimental signals from anti-vWF IgGs on the nanoanchors. The blue curve 1 is a weighted fit of the measured probability density function for sparsely distributed IgGs on glass and blue curves 2, 3, 4 etc. are the weighted extrapolations for 2, 3, 4 etc. IgGs, respectively. The red curve is the sum of the blue curves. The weighting indicates that 34% of the nanoanchors carry exactly one antibody, 20% carry two, 21% carry three, 10% carry four and 15% carry five or more fluorescent IgG antibodies

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