Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: STED lithography in microfluidics for 3D thrombocyte aggregation testing

Fig. 1

Microfluidic device for thrombocyte activation studies. a Sketch representing side- and top-view of the microfluidic device. Blue: glass slides, orange: double-sided adhesive tape with a thickness of 83.1 µm, green: in- and outlet connectors. b Scanning electron microscopy (SEM) images of the structures inside the microfluidic channel. Protein repellent MPL grids on posts (grey) carry STED-lithography written nanoanchors (green). c Left: Sketch of the protein repellent structure (grey) with protein binding nanoanchors (green). Right: Fluorescence microscopy image of an empty scaffold. Excitation wavelength: 491 nm, illumination time 5 ms. d Left: Sketch of the experiment for thrombocyte activation. The protein binding nanoanchors were labeled with von Willebrand factor (vWF) molecules. Subsequently, thrombocytes were added to the microfluidic device. Fluorescently labeled antibodies (a-CD62P Alexa 647) were bound to the activated thrombocytes. Right: Fluorescence image of activated thrombocytes bound on top of the scaffolds. Excitation wavelength: 642 nm, illumination time 5 ms. e Left: Sketch of the control experiment where vWF molecules were omitted. The structures were incubated with a thrombocyte concentrate under flow conditions and subsequently incubated with fluorescently labeled a-CD62p antibodies. Right: There were no activated thrombocytes present on the structures. Excitation wavelength: 642 nm, illumination time 5 ms

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