Table 1 The strains used in this study

From: Direct RBS Engineering of the biosynthetic gene cluster for efficient productivity of violaceins in E. coli

Strain Characteristic Source
C. violaceum ATCC 12472 Violaceins natural producer BNCC
E. coli DH5α Clone host strain Invitrogen
E. coli BL21(DE3) Protein expression host strain Invitrogen
E. coli BL21(DE3) (tnaA) tnaA gene was knocked out in E. coli BL21(DE3) This study
E. coli BL21(DE3) (Vio12472) The plasmid Vio12472 in E. coli BL21(DE3) This study
E. coli BL21(DE3) (dVio12472) The plasmid dVio12472 in E. coli BL21(DE3) This study
Bm-1 The plasmid Vio12472-vioB-RBSm-1 in E. coli BL21(DE3) This study
Bm-2 The plasmid Vio12472-vioB-RBSm-2 in E. coli BL21(DE3) This study
Bm-3 The plasmid Vio12472-vioB-RBSm-3 in E. coli BL21(DE3) This study
Cm The plasmid Vio12472-vioC-RBSm in E. coli BL21(DE3) This study
Dm-1 The plasmid Vio12472-vioD-RBSm-1 in E. coli BL21(DE3) This study
Dm-2 The plasmid Vio12472-vioD-RBSm-2 in E. coli BL21(DE3) This study
Em The plasmid Vio12472-vioE-RBSm in E. coli BL21(DE3) This study
(BC)m The plasmid Vio12472-vioBC-RBSm in E. coli BL21(DE3) This study
(BD)m The plasmid Vio12472-vioBD-RBSm in E. coli BL21(DE3) This study
(BE)m The plasmid Vio12472-vioBE-RBSm in E. coli BL21(DE3) This study
(CD)m The plasmid Vio12472-vioCD-RBSm in E. coli BL21(DE3) This study
(CE)m The plasmid Vio12472-vioCE-RBSm in E. coli BL21(DE3) This study
(DE)m The plasmid Vio12472-vioDE-RBSm in E. coli BL21(DE3) This study
(BCD)m The plasmid Vio12472-vioBCD-RBSm in E. coli BL21(DE3) This study
(BCE)m The plasmid Vio12472-vioBCE-RBSm in E. coli BL21(DE3) This study
(BDE)m The plasmid Vio12472-vioBDE-RBSm in E. coli BL21(DE3) This study
(CDE)m The plasmid Vio12472-vioCDE-RBSm in E. coli BL21(DE3) This study
(BCDE)m The plasmid Vio12472-vioBCDE-RBSm in E. coli BL21(DE3) This study
(BCDE)m (tnaA) The plasmid Vio12472-vioBCDE-RBSm in E. coli BL21(DE3) (tnaA) This study
Vio12472 (tnaA) The plasmid Vio12472 in E. coli BL21(DE3) (tnaA) This study