Fig. 5 | Microbial Cell Factories

Fig. 5

From: Direct RBS Engineering of the biosynthetic gene cluster for efficient productivity of violaceins in E. coli

Fig. 5

The fermentation titers of the strains in the third and fourth round of RBS mutagenesis and (BCDE)m optimized by one-factor-at-one-time method. a The titers of (BCD)m, (BCE)m, (BDE)m, (CDE)m and (BCDE)m compared with the wild type strain E. coli BL21(DE3) (Vio12472). b The fermentation titers of (BCDE)m at different temperatures (16 ℃, 20 ℃, 25 ℃, 30 ℃, 37 ℃). c The fermentation titers of (BCDE)m at various concentrations of IPTG (0.01, 0.02, 0.04, 0.06, 0.08, 0.10, 0.20, 0.40, 0.60, 0.80 and 1.00 mM). (D) The fermentation titers for violacein and deoxyviolacein produced by (BCDE)m in the 192 h’s cultivation period

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