Fig. 3 | Respiratory Research

Fig. 3

From: Chronic intermittent hypoxia promoted lung cancer stem cell-like properties via enhancing Bach1 expression

Fig. 3

CIH promoted CSC-like properties in NSCLC cells. a Microscopic observation of NSCLC cells. A549 and SPCA1 spheroids were obtained and then cultured under Nor or CIH conditions for 24 h. b qRCP analyses in triplicate of CD44, Sox2, Nanog, Oct4, and CD133 in NSCLC cells. Adherent A549 and SPCA1 were treated with 24 h-Nor or CIH exposure. GADPH expression served as an internal control. c Flow cytometry analyses of the percentage of CD44+CD133+ cells in A549 or SPCA1 populations. Error bars represent the mean ± SEM of at least triplicate experiments. *P < 0.05, **P < 0.01. CIH chronic intermittent hypoxia, CSC cancer stem cell

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