Fig. 2 | Respiratory Research

Fig. 2

From: Chronic intermittent hypoxia promoted lung cancer stem cell-like properties via enhancing Bach1 expression

Fig. 2

CIH promoted lung cancer cell proliferation. Cell validations of A549 (a) and SPCA1 (b) were detected by CCK-8 assay after exposure to Nor or CIH for 24, 48, 72, and 96 h. Cell proliferation of A549 (c) or SPCA1 (d) after 48-h Nor or CIH exposure was assessed using Edu assay. e The percentage of Edu positive cells in A549 or SPCA1 was analyzed and illustrated. f, g Assessment of cancer genericity was performed by colony formation analysis after CIH treatment. h The number of colonies was measured and illustrated. Error bars represent the mean ± SEM of at least triplicate experiments. *P < 0.05, **P < 0.01. CIH chronic intermittent hypoxia

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