Fig. 2 | Respiratory Research

Fig. 2

From: GITRL on dendritic cells aggravates house dust mite-induced airway inflammation and airway hyperresponsiveness by modulating CD4+ T cell differentiation

Fig. 2

In vivo GITRL is successfully knocked down by intranasal delivery of the AAV vector. a Immunofluorescent staining for GITRL in AAV-GFP- and AAV-shGITRL-transduced mice (× 200 magnification, scale bars = 50 μm). b Quantification of the GITRL mean fluorescent intensity (MFI) in the lung (n = 5 per group). c Representative western blots of GITRL protein in the lungs of AAV-GFP- and AAV-shGITRL-transduced mice. d Quantification of western blot data from (c) (n = 5 per group). e Flow cytometry analysis of the percentage of CD11c+MHCII+GITRL+ cells in the lung tissues from HDM-challenged AAV-GFP and AAV-shGITRL mice. f Statistical analysis of (e) (n = 4 per group). *P < 0.05, **P < 0.01

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