Fig. 1 | Respiratory Research

Fig. 1

From: GITRL on dendritic cells aggravates house dust mite-induced airway inflammation and airway hyperresponsiveness by modulating CD4+ T cell differentiation

Fig. 1

Increased GITRL expression on lung dendritic cells from HDM-induced asthmatic mice. a Mice were sensitized by intranasal house dust mite (HDM) administration (20 μg) on day 0 and day 14 and challenged by intranasal HDM (20 μg) administration on days 21, 23, 25, 27 and 29 before sacrifice on day 31. b GITRL mRNA expression in the lung tissues of normal saline and HDM-challenged mice. c Representative western blots of GITRL protein in the lung tissues of normal saline and HDM-challenged mice. d Quantification of western blots data from (c) (n = 8 per group). e Immunohistochemical staining for GITRL in the lung tissues of normal saline and HDM-challenged mice (× 200 magnification, scale bars = 500 μm). f The total integral optical density (IOD) for GITRL (n = 9 per group). g Flow cytometry analysis of the percentage of CD11c+MHCII+GITRL+ cells in the lung tissues of normal saline and HDM-challenged mice. h Statistical analysis of (g) (n = 8 per group). *P < 0.05, **P < 0.01

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