Table 3 Box-Behnken design (matrix and responses) for levan production by BD1707

From: Levan from Leuconostoc citreum BD1707: production optimization and changes in molecular weight distribution during cultivation

Run A B C D Levan production (g/L)
Experimental Predicted
1 −1 0 1 0 31.72 31.82
2 0 0 0 0 33.40 33.36
3 0 0 −1 −1 21.92 22.47
4 1 0 −1 0 33.16 32.87
5 0 −1 0 −1 11.46 10.30
6 1 1 0 0 27.88 28.47
7 0 0 0 0 32.94 33.36
8 0 0 1 1 30.97 30.62
9 −1 0 −1 0 27.28 28.42
10 0 1 0 −1 19.94 19.84
11 0 1 −1 0 28.77 27.76
12 1 0 1 0 31.44 30.10
13 0 1 1 0 27.95 27.50
14 −1 −1 0 0 17.60 17.21
15 −1 0 0 1 29.96 29.69
16 0 −1 −1 0 16.84 17.29
17 −1 1 0 0 27.36 27.37
18 1 0 0 1 31.80 32.38
19 1 −1 0 0 18.64 18.84
20 0 1 0 1 27.08 28.04
21 0 0 1 −1 22.30 23.34
22 1 0 0 −1 22.96 23.22
23 −1 0 0 -1 23.76 23.17
24 0 -1 1 0 17.18 18.18
25 0 -1 0 1 17.88 17.79
26 0 0 0 0 33.74 33.36
27 0 0 -1 1 31.71 30.87