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Table 3 Comparison of diagnostic performance of mNGS and CMTs in immunocompromised patients

From: Diagnostic value of metagenomic next-generation sequencing of bronchoalveolar lavage fluid for the diagnosis of suspected pneumonia in immunocompromised patients

  CMTs mNGS
  Sensitivity%
(95% CI)
Specificity%
(95% CI)
PPV%
(95% CI)
NPV%
(95% CI)
Accuracy%
(95% CI)
Sensitivity%
(95% CI)
Specificity%
(95% CI)
PPV%
(95% CI)
NPV%
(95% CI)
Accuracy%
(95% CI)
Bacterial pneumonia 36 (21–50) 82 (73–90) 53 (31–74) 69 (62–76) 65 (54–76) 80 (63–92) 73 (63–79) 63 (49–72) 87 (75–94) 75 (63–84)
Fungal pneumonia 50 (37–62) 63 (50–75) 57 (42–71) 54 (45–67) 57 (44–69) 91 (79–98) 66 (54–72) 72 (63–77) 89 (73–97) 78 (66–85)
Pneumocystis jiroveciia 28 (16–28) 100 (91–100) 100 (58–100) 71 (66–71) 74 (66–74) 100 (86–100) 86 (79–86) 81 (70–81) 100 (90–100) 91 (82–91)
Pneumocystis jiroveciib 88 (72–97) 84 (75–89) 76 (62–83) 93 (83–98) 86 (74–92) 100 (86–100) 86 (79–86) 81 (70–81) 100 (90–100) 91 (82–91)
Aspergillus spp. 86 (63–99) 97 (92–98) 75 (41–87) 98 (94–100) 96 (88–98) 57 (23–71) 98 (95–100) 80 (33–99) 95 (92–97) 94 (87–97)
Viral pneumonia 53 (34–53) 100 (95–100) 100 (64–100) 89 (84–89) 90 (81–90) 93 (70–100) 83 (77–85) 61 (46–65) 98 (90–100) 86 (75–88)
  1. mNGS metagenomic next-generation sequencing, CMTs conventional microbiological tests, PPV positive predictive value, NPV negative predictive value
  2. aDetect by Gomori methenamine silver staining only
  3. bDetect by a combination of Gomori methenamine silver staining and serum (1,3)-b-D-glucan (≥ 80 ng/l was defined as positive)