Skip to main content

Table 5 Primer sequence in the RT-PCR

From: Revealing potential diagnostic gene biomarkers of septic shock based on machine learning analysis

Primer name Primer sequence (5ʹ to 3ʹ)
GAPDH-F (Internal reference) 5-CTGGGCTACACTGAGCACC-3
GAPDH-R (Internal reference) 5-AAGTGGTCGTTGAGGGCAATG-3
ACTB-F (Internal reference) 5-TCCGCAAAGACCTGTACGC-3
ACTB-R (Internal reference) 5-CTGGAAGGTGGACAGCGAG-3
ARHGEF18-F 5-ACGCCAGCAAAGAAGACGT-3
ARHGEF18-R 5-CAGGCGGTCATCAGTGGTT-3
CLEC5A-F 5-GCAATTGTCAACACGCCAGA-3
CLEC5A-R 5-GCCAATGGTCGCACAGTTG-3
DUSP3-F 5-GAAGATATGGGGCAACTGGA-3
DUSP3-R 5-ATGCACGTGTTCAGCTTGAG-3
FCER1A-F 5-GTTCTTCGCTCCAGATGGC-3
FCER1A-R 5-TTGTGGAACCATTTGGTGGAA-3
HDAC4-F 5-AGCGTCCGTTGGATGTCAC-3
HDAC4-R 5-CCTTCTCGTGCCACAAGTCT-3
KLRF1-F 5-GGGAATATCTGGAACCGTGA-3
KLRF1-R 5-CTGCAGATCTCGAAGCACAA-3
UPP1-F 5-TGGAGTCCTCGGTGTTTGC-3
UPP1-R 5-GCTCAGGCCTTGCTCAGTT-3