Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot
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The induction of alcohol fermentation in roots is a plant adaptive response to flooding stress and oxygen deprivation. Available transcriptomic data suggest that fermentation-related genes are also frequently induced in roots infected with gall forming pathogens, but the biological significance of this induction is unclear. In this study, we addressed the role of hypoxia responses in Arabidopsis roots during infection by the clubroot agent Plasmodiophora brassicae.
The hypoxia-related gene markers PYRUVATE DECARBOXYLASE 1 (PDC1), PYRUVATE DECARBOXYLASE 2 (PDC2) and ALCOHOL DEHYDROGENASE 1 (ADH1) were induced during secondary infection by two isolates of P. brassicae, eH and e2. PDC2 was highly induced as soon as 7 days post inoculation (dpi), i.e., before the development of gall symptoms, and GUS staining revealed that ADH1 induction was localised in infected cortical cells of root galls at 21 dpi. Clubroot symptoms were significantly milder in the pdc1 and pdc2 mutants compared with Col-0, but a null T-DNA insertional mutation of ADH1 did not affect clubroot susceptibility. The Arg/N-end rule pathway of ubiquitin-mediated proteolysis controls oxygen sensing in plants. Mutants of components of this pathway, ate1 ate2 and prt6, that both exhibit constitutive hypoxia responses, showed enhanced clubroot symptoms. In contrast, gall development was reduced in quintuple and sextuple mutants where the activity of all oxygen-sensing Group VII Ethylene Response Factor transcription factors (ERFVIIs) is absent (erfVII and prt6 erfVII).
Our data demonstrate that the induction of PDC1 and PDC2 during the secondary infection of roots by P. brassicae contributes positively to clubroot development, and that this is controlled by oxygen-sensing through ERFVIIs. The absence of any major role of ADH1 in symptom development may also suggest that PDC activity could contribute to the formation of galls through the activation of a PDH bypass.
KeywordsEthanol fermentation Plant gall disease Clubroot Plasmodiophora Arabidopsis ADH1 PDC2 N-end rule pathway Hypoxia ERFVII
Arginine-trna protein transferase 1
Arginine-trna protein transferase 2
Days post inoculation
Group VII Ethylene Response Factor transcription factors
Pyruvate decarboxylase 1
Pyruvate decarboxylase 2
Related to apetala
Clubroot is a root gall disease of Brassicaceae species, caused by the protist Plasmodiophora brassicae. The infection process involves a short primary infection of root hairs by zoospores, followed by a secondary phase where plasmodia develop intracellularly in the root cortex for several weeks. During this secondary phase, P. brassicae induces hypertrophia and hyperplasia of infected plant cortical cells, leading to the development of galls and to the wilting of the infected plant .
Functional genomics approaches have established an increasingly detailed picture of plant signaling and metabolic pathways involved in positive or negative control of clubroot gall development . Untargeted transcriptomic analyses also highlighted additional mechanisms of regulation, but the biological significance of many of these remains uncertain. In this context, Jubault et al.  and Schuller et al.  pinpointed the induction of ethanol fermentation during secondary infection by P. brassicae, and both studies suggested that ethanol fermentation may allow root cells to cope with an oxygen deficit induced by tumor development or by the increased energetic demand in infected cells.
Ethanol fermentation, i.e., conversion of pyruvate into ethanol by the action of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH), is the classical hallmark of root responses to flooding. Under limited oxygen conditions, fermentation allows plant cells to avoid toxic accumulation of pyruvate that would result from the decrease in mitochondrial respiratory activity. This process allows cells to sustain glycolytic fluxes and to meet minimal energetic and metabolic needs to cope with moderate hypoxia constraints. In Arabidopsis thaliana, ADH1, PDC1 and PDC2 have been reported to be important players in flooding-triggered fermentation responses [5, 6, 7]. Hypoxia is sensed in plants through the N-end rule pathway of ubiquitin-mediated targeted proteolysis [8, 9]. The five Group VII Ethylene Response Factor transcription factors (RELATED TO APETALA [RAP]2.12, RAP2.2, RAP2.3, HYPOXIA RESPONSIVE ERF [HRE]1 and HRE2) are the only known plant substrates of this pathway, and their oxygen-dependent degradation controls the hypoxia-associated expression of fermentation genes. Oxidation of amino-terminal Cysteine (OXCys) of ERFVII proteins in vivo by oxygen (and nitric oxide) leads to amino-terminal arginylation of OXCys by ARGINYL TRANSFERASES (ATEs) that allows recognition by an E3 ligase of the N-end rule pathway PROTEOLYSIS (PRT)6 and subsequent ubiquitination and degradation [10, 11, 12].
The objectives of the present study were to: 1) specifically document the temporal regulation of ethanol fermentation and other hypoxia-responses during pathogen-induced gall development, and 2) assess the extent to which fermentation, hypoxia-sensing and responses may contribute to the enhancement or reduction of tumorigenic processes. The induction of fermentation during clubroot development was assessed by a combination of RT-qPCR analysis, GUS staining and respiration measurements. The development of clubroot symptoms was evaluated in mutant lines defective for ethanol fermentation, and in mutants of the N-end rule pathway exhibiting constitutive induction (in mutants of the E3 ligase; prt6 or Arginyl transferase ate1 ate2) or constitutive absence of hypoxia responses (erfVII and prt6 erfVII). The expression of previously described N-end rule and hypoxia regulated genes available in several transcriptome datasets from studies on different tumour-inducing pathogens (P. brassicae, the root knot nematode Meloidogyne javanica and the crown gall agent Agrobacterium tumefaciens) was assessed.
Hypoxia-responsive genes are induced early following P. brassicae infection, and amplified with club development
GUS staining revealed the induction of ADH1 in cortical cells of developed root clubs infected by P. brassicae
Root respiration activity is not affected during the early secondary phase of clubroot infection
Removal of PCD1 and PDC2 function reduces the development of clubroot
Constitutive activation or repression of hypoxia responses in A. thaliana mutant lines resulted in enhanced or repressed development of clubs
Hypoxia-transcriptional fingerprints are commonly induced in tumorigenic plant pathogen interactions
ADH1 and PDC1 are commonly used as marker genes for the study of hypoxia responses in plants. The induction of these genes is the emerged face of a (small) iceberg of co-regulated genes that are collectively controlled by the Arg/N-end rule pathway [8, 16]. The data presented in the present work converge to the idea that P. brassicae infection significantly induces ADH1, PDC1 and PDC2 during the secondary infection of roots, and that this response should be viewed as a component of a global stereotypical hypoxia response. Two major factors may induce genuine hypoxia in clubroot infected root tissues: First, the oxygen diffusion rate may be significantly reduced in tumorigenic tissues (previously proposed by  and ). Second, keeping in mind the observation that P. brassicae plasmodia develop intracellularly inside root cortical cells, the plant hypoxia response may result from an intracellular competition for oxygen between the respective mitochondria of Arabidopsis and Plasmodiophora. Both hypotheses would be consistent with the localisation of ADH1::GUS staining in the core infected cells of the root galls (Fig. 2). In our data however, hypoxia-response gene induction was found as soon as 7 dpi, i.e. a time point at the very beginning of root cortical infection where galls are not yet visible. Then, for the earliest time point of the secondary infection, hypoxia responses may result from subtle or localized drops of oxygen availability. Alternatively, one can also not exclude that this response may be triggered by the modulation of other plant-derived factors such as nitric oxide, that also affects Arg/N-end rule degradation of ERFVIIs . If plant hypoxia responses are of benefit to pathogen development, it should be also worthy to envisage that the Arg/N-end rule pathway could be influenced by biochemical effectors of the pathogen, as previous work has shown that this pathway is regulated by small molecules . Additional work would be needed for a clarification of these different possibilities.
Our principal objective in this study was to identify the biological consequences of the induction of fermentative metabolism during clubroot infection. We therefore used appropriate mutant lines to clarify who from the host plant and/or the pathogen would be the payee of ethanol fermentation. The Arabidopsis genome harbours four different PDC encoding genes, but to date only PDC1 and PDC2 have been reported to play significant role in hypoxia and flooding responses [5, 6, 7]. In the present work, the phenotypes of pdc1 and pdc2 mutants indicate that both genes positively contribute to the development of clubroot symptoms. This clearly eliminates the conceivable hypothesis that ethanol biosynthesis by plant cells could act as an antibiotic for inhibiting P. brassicae development. Rather, the phenotypes of pdc and Arg/N-end rule pathway mutants support a model where hypoxia response benefits disease development and pathogen spore production. This response may be originally a response of the plant to cope with the reduced oxygen availability caused by the infection. As a ‘secondary effect’, the metabolic adaptation to hypoxia may benefit the pathogen, just because any biotrophic pathogen benefits from a host plant that can maintain its metabolic functions as much as possible during the infection process. As discussed above, this conclusion requires confirmation that cell oxygen content actually drops during the development of clubs.
We previously reported  that clubroot disease development reaches higher rates in Col-0 when infected plants are cultivated in well-aerated soil substrate. Waterlogging led to the inhibition and restriction of gall development on plant collars, i.e. above the level of water-saturated soil. Thus, from the present study, conducted in well-aerated substrate, it may be inferred that clubroot development is paradoxically at its maximum when hypoxia response is induced in well-aerated infected roots.
ADH activity is a major step of ethanol fermentation because this step allows the regeneration of NAD+, thereafter supporting intensive glycolysis flux, and the resulting production of ATP, when respiration is impaired. ADH1, being the only ADH encoding gene in Arabidopsis, is actually a key gene to support this mechanism in cells under anoxia . The absence of difference for clubroot symptoms between the adh1-4 mutant and the wild type was surprising and suggests that, beyond transcriptional regulation, clubroot infection may not activate a genuine anaerobic ethanol fermentation response: 1/ regeneration of NAD+ might not be a major stake in root cells infected by P. brassicae 2/ PDC-derived acetaldehyde undergoes non-ethanolic fates. Such metabolic features are reminiscent of the ‘PDH bypass’ model, a metabolic pathway also reported as ‘aerobic fermentation’, where acetaldehyde produced by the decarboxylation of pyruvate, is converted to acetyl-CoA, thus furnishing the biosynthesis of fatty acids . The PDH bypass has been experimentally documented in aerobic plant tissues [25, 26], and has been proposed to play a role in the rapid development of actively respiring sporophytic tissues during pollen germination . In the context of clubroot infection, this mechanism would fit with above-described unexpected data: 1/ the fermentation response is triggered at a time point where respiration is apparently unaffected in infected roots 2/ clubroot symptoms are similar in the adh1-4 mutant and in the wild type. This model would also make sense with the recently reported auxotrophy of P. brassicae for fatty acids, suggested by the absence of fatty acid synthase in its genome . Thus, for an efficient clubroot infection, the pathogen may require the activation of metabolic plant features, possibly including a PDH-bypass, which would allow massive synthesis of acetyl-CoA for the synthesis of fatty acids. A careful investigation on carbon fluxes in a series of appropriate mutants would be necessary to test this hypothesis.
Ethanol fermentation in plant cells has been mostly studied for its role in flooding and hypoxia/anoxia responses. The present work shows that pyruvate decarboxylase genes PDC1 and PDC2 support the development of clubroot, and increase the fitness of pathogen through enhancing spore production. The induction of ethanol fermentation genes is part of a prototypical Arg/N-end rule driven hypoxia response, controlled by ERFVII transcription factors, which may play a role in the infection of many gall-forming pathosystems. Further work is needed to assess if hypoxia actually drives the response during the earliest steps of the clubroot infection, and to test the possible role of PDH bypass in regulating clubroot development.
All mutant lines were in the Arabidopsis genetic background Columbia. The confirmed homozygous mutant line pdc1 (SALK_090204C, ) harbours a T-DNA in the second exon of the gene At4g33070, and was obtained from NASC. The mutant line pdc2 (SAIL_650_C05) harbours a homozygous insertion in the unique exon of the gene At5G54960, and was obtained from NASC (N862662). The mutant adh1-4 mutant, obtained from NASC (N66116), harbours a knock-out mutation in the gene At1g77120 generated through Zinc Finger Nuclease as described in . The promADH1::GUS line (described in ) was kindly provided by Dr. Robert J. Ferl (University of Florida, USA). Arg/N-end rule pathway and erfVII mutants were described previously [8, 17].
Clubroot assays were performed as previously described in , using isolates eH and e2 of P. brassicae described in . Experiments were performed with 3 or 4 independent biological replicates, as specified in the figure legends. Each replicate consisted of at least 12 individual plants and relative spatial disposition of genotypes was randomized in every biological replicate to avoid possible positional effects. All sampled plants were briefly washed with tap water, and then photographed for the evaluation of disease symptoms through image analysis with ImageJ software, as described in . A disease index was calculated as the ratio between the gall area (Ga, in cm2) and the square of the longest leaf length (La, in cm2) of the rosette, and this ratio was multiplied by a factor of 5000. For each replicate, all of the individual root samples were pooled for further spore quantification of RNA extraction. Spore content in infected roots was evaluated with a flow cytometer as described previously in .
RT- qPCR experiments
The expression of hypoxia-responsive genes ADH1 (At1g77120), PDC1 (At4g33070) and PDC2 (At5G54960) was monitored in the roots of Col-0 under three experimental conditions: 1) non-inoculated, 2) inoculated with isolate eH, and 3) inoculated with isolate e2. Root samples were collected at two time-points (7 and 17 days post-inoculation, dpi), and then immediately frozen in liquid nitrogen prior to storage at −80 °C. RNA extraction and reverse transcription were performed according to , using PP2A3 (At1G13320) as housekeeping reference gene. The primers were as follows: PP2AFor-TAACGTGGCCAAAATGATGC/ PP2ARev-GTTCTCCACAACCGCTTGGT/ PDC1.2For-GGTGGAAGCAACATTGGAGT/ PDC1.2Rev-GCTCACTGCTCCCCAATAAG/ PDC2.2For-TTGAGGCCATACACAATGGA / PDC2.2Rev-GGATTTGGGGGACGACTATT/ ADH1.2For-GGTCTTGGTGCTGTTGGTTT/ ADH1.2Rev-CTCAGCGATCACCTGTTGAA.
The promADH1::GUS line was challenged with isolate eH and e2 in a bioassay as described above. Plants were sampled at 7 and 21 dpi, and GUS staining (overnight incubation) and histological observations were performed following . To obtain a positive control of promADH1::GUS expression, a set of non-inoculated plants was sampled at 6 dpi and maintained in hypoxia conditions for 24 additional hours by dipping inside 50 mL tubes full-filled with tap water, before staining.
Respiration was evaluated in samples of roots from plants at 7 dpi using an oxygraph-2 K (Oroboros). The tank of the oxygraph was filled with deionised water and saturated with oxygen by air bubbling. Measurements were calibrated based on room temperature and atmospheric pressure in each experiment. Root samples were immersed in the tank, with a gentle stirring to ensure proper agitation of the medium. The tank was then filled to capacity with additional water to avoid any remaining volume of air above the water. The decrease in oxygen concentration in the water was monitored over a 5 min period. The resulting rate of oxygen consumption was divided by the fresh biomass of the roots.
Jean-Michel Lequéré (INRA, UR 0117 URC) is acknowledged for helpful discussions and precious technical support with oxygraph measurements. All colleagues from IGEPP who brought their help for the sampling of infected plants are warmly acknowledged.
SL was supported by a CJS grant from the National Institute for Agronomic Research (INRA). ARS was supported by a Marie Curie FP7 fellowship. MJH and JV were supported by BBSRC grants BB/K000144/1 and BB/M029441/1 (including financial support from SABMiller plc). This work also benefited from core funding from AGROCAMPUS Ouest, INRA and Université de Rennes 1.
Availability of data and materials
All supporting data can be found within the manuscript.
AG/MJM/MJH designed the study with the help of all other co-authors, AG/GR/TL/SL/ARS/CL/JL/JV/MJ performed the experimental work and AG/GR/MJM/MJH wrote the manuscript. All authors have read and approved this manuscript.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 3.Jubault M, Lariagon C, Taconnat L, Renou J-P, Gravot A, Delourme R, et al. Partial resistance to clubroot in Arabidopsis is based on changes in the host primary metabolism and targeted cell division and expansion capacity. Funct Integr Genomics. 2013;13:191–205.CrossRefPubMedPubMedCentralGoogle Scholar
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