Neutral lipid analysis of substitution mutants. TAG and SE content of the AWRI1631 (panel a) and the BY4741 (panel b) mutant strains. The genes PIG1, PHO23 or RML2 and combinations of these genes are replaced with the alleles from the other parent strain. The mutants were cultivated in minimal medium for 48 h. The data are the means from a minimum of three independent experiments and their standard deviations. The p-values are the results of a two-tailed t-test comparing the respective mutant with the wild-type.