Neutral lipid analysis of deletion mutants. TAG and SE content of the AWRI1631 (panel a) and the BY4741 (panel b) strains, deleted for PIG1, PHO23 or RML2, and combinations thereof. These results confirm that the proteins encoded by these three genes are involved in NL metabolism, with varying influence in the two strain backgrounds. The strains were cultivated in minimal medium for 48 h. The data are the means from a minimum of three independent experiments and their standard deviations. The p-values are the results of a two-tailed t-test comparing the respective mutant with the wild-type.