Table 1 Summary of antibodies used in immunocytochemistry and western blotting

From: Targeting BMI-1 with PLGA–PEG nanoparticle-containing PTC209 modulates the behavior of human glioblastoma stem cells and cancer cells

Primary antibody Cell target Dilution Clonality Host species Company Secondary antibody
Ki67 Proliferation marker 1:100 Polyclonal Rabbit Abcam Goat
Nestin Neural stem cells 1:50 Monoclonal Mouse Santa Cruz Goat
GFAP Glial Tumor cells 1:300 Monoclonal Mouse Sigma Goat
CD34 Endothelial cells 1:20 Monoclonal Human eBioscience Mouse
CD44 Cancer progenitor cells 1:50 Monoclonal Human eBioscience Rat
CD45 Hematopoietic stem cells 1:20 Monoclonal Mouse eBioscience Goat
NF-kB-p65 Mammalian cells 1:1000 Polyclonal Rabbit Abcam Goat
p-Akt Mammalian cells 1:1000 Polyclonal Rabbit Cell signaling Goat
p-SAPK/JNK Mammalian cells 1:1000 Polyclonal Rabbit Cell signaling Goat
β-Actin Mammalian cells 1:1000 Monoclonal Rabbit Cell signaling Goat