Fig. 7 | Cancer Nanotechnology

Fig. 7

From: Targeting BMI-1 with PLGA–PEG nanoparticle-containing PTC209 modulates the behavior of human glioblastoma stem cells and cancer cells

Fig. 7

The effects of Nano-PTC209 on apoptosis and cell cycle in glioblastoma stem-like cells (GSCs) and U87MG (U87). a Annexin V/propidium iodide (PI) double-stained was used to determine the apoptosis of GSCs and U87 cells treated with different concentrations of Nano-PTC209. Untreated cells were indicated as control. Diagram quarters are corresponding to live cells (Q4), early apoptotic (Q3), late apoptotic (Q2), and necrotic cells (Q4). Both GSCs and U87 cells treated with Nano-PTC209 exhibited a higher amount of apoptotic and necrotic cells. b The effects of different concentrations of Nano-PTC209 on the cell cycle of GSCs and U87 cells were assessed. The cells were stained with propidium iodide and the cell cycle was measured using flow cytometry. Cell cycle analysis revealed that Nano-PTC209 at doses of 137 nM resulted in a higher accumulation of U87 cells in the G0/G1 phase (P < 0.05). c Effect of Nano-PTC209 application on p53 mRNA expression in GSCs and U87 cells. Nano-PTC209 increased the expression of p53 in both GSCs and U87 cells. Data are expressed as the mean ± SD. ** and *** indicates P < 0.01 and P < 0.001, respectively

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