Conjugation Magnetic PAEEP-PLLA Nanoparticles with Lactoferrin as a Specific Targeting MRI Contrast Agent for Detection of Brain Glioma in Rats
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The diagnosis of malignant brain gliomas is largely based on magnetic resonance imaging (MRI) with contrast agents. In recent years, nano-sized contrast agents have been developed for improved MRI diagnosis. In this study, oleylamine-coated Fe3O4 magnetic nanoparticles (OAM-MNPs) were synthesized with thermal decomposition method and encapsulated in novel amphiphilic poly(aminoethyl ethylene phosphate)/poly(L-lactide) (PAEEP-PLLA) copolymer nanoparticles. The OAM-MNP-loaded PAEEP-PLLA nanoparticles (M-PAEEP-PLLA-NPs) were further conjugated with lactoferrin (Lf) for glioma tumor targeting. The Lf-conjugated M-PAEEP-PLLA-NPs (Lf-M-PAEEP-PLLA-NPs) were characterized by photon correlation spectroscopy (PCS), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), thermo-gravimetric analysis (TGA), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). The average size of OAM-MNPs, M-PAEEP-PLLA-NPs, and Lf-M-PAEEP-PLLA-NPs were 8.6 ± 0.3, 165.7 ± 0.6, and 218.2 ± 0.4 nm, with polydispersity index (PDI) of 0.185 ± 0.023, 0.192 ± 0.021, and 0.224 ± 0.036, respectively. TEM imaging showed that OAM-MNPs were monodisperse and encapsulated in Lf-M-PAEEP-PLLA-NPs. TGA analysis showed that the content of iron oxide nanoparticles was 92.8 % in OAM-MNPs and 45.2 % in Lf-M-PAEEP-PLLA-NPs. VSM results indicated that both OAM-MNPs and Lf-M-PAEEP-PLLA-NPs were superparamagnetic, and the saturated magnetic intensity were 77.1 and 74.8 emu/g Fe. Lf-M-PAEEP-PLLA-NPs exhibited good biocompatibility in cytotoxicity assay. The high cellular uptake of Lf-M-PAEEP-PLLA-NPs in C6 cells indicated that Lf provided effective targeting for the brain tumor cells. The T 2 relaxation rate (r 2) of M-PAEEP-PLLA-NPs and Lf-M-PAEEP-PLLA-NPs were calculated to be 167.2 and 151.3 mM−1 s−1. In MRI on Wistar rat-bearing glioma tumor, significant contrast enhancement could clearly appear at 4 h after injection and last 48 h. Prussian blue staining of the section clearly showed the retention of Lf-M-PAEEP-PLLA-NPs in tumor tissues. The results from the in vitro and in vivo MRI indicated that Lf-M-PAEEP-PLLA-NPs possessed strong, long-lasting, tumor targeting, and enhanced tumor MRI contrast ability. Lf-M-PAEEP-PLLA-NPs represent a promising nano-sized MRI contrast agent for brain glioma targeting MRI.
KeywordsPoly(aminoethyl ethylene phosphate)/poly (L-lactide) copolymer Oleylamine-coated Fe3O4 nanoparticles Lactoferrin Superparamagnetic MRI contrast agent Brain glioma targeting
rat C6 glioma cell
Fourier transform infrared
hematoxylin and eosin
human normal liver cell
magnetic resonance imaging
oleylamine-coated Fe3O4 magnetic nanoparticles
poly(aminoethyl ethylene phosphate)/poly(L-lactide)
susceptibility weighted imaging.
transmission electron microscopy
Malignant glioma is a primary brain tumor that originates from the supportive cells of the brain called glial cells. Because of the high proliferation of blood vessels and endothelial cells, a major pathophysiological feature of gliomas is their ability to diffuse and invade into surrounding brain tissue . Although there are significant advances in neuroimaging and neurosurgical techniques over the past 30 years, the median survival time of glioblastoma patients is only 12 to 18 months after surgery [2, 3].
The diagnosis of malignant gliomas is largely based on radiographic findings in the setting of a typical clinical presentation and biopsy specimen obtained invasively . Magnetic resonance imaging (MRI), a much easier, safer, and non-invasive tool, has been widely used in clinical diagnosis . MRI is very effective in characterizing soft tissue because of its high tissue resolution . Current approach for diagnosis and grading of brain glioma is MRI with administration of gadolinium (Gd) chelate-based contrast agents . However, such agents have several shortcomings, such as short imaging time, fast diffusion, and side effect of nephrogenic systemic fibrosis. And Gd-enhanced T 1-weighted MRI images could not distinguish effectively various etiologies, such as tumor regrowth and radiation necrosis .
In recent years, superparamagnetic iron oxide nanoparticles (SPIONs) have been developed as MRI contrast agents for diagnosis of malignant gliomas . SPIONs are usually less than 10 nm, which are useful as T 2 contrast agents owning to more effective at shortening T 2 rather than T 1 relaxation time. SPIONs can generate contrast signals at higher level than paramagnetic Gd chelates, providing an opportunity for the early detection of tumors, and SPIONs display outstanding biocompatibility and biodegradability . Current approaches to developing SPION-based MRI contrast agents with long-time stability and specificity to target tumor are to incorporate iron oxide into polymeric nanocarriers [10, 11]. The polymeric nanocarriers with incorporation of iron oxide revealed higher relaxivity than dissociated monocrystalline iron oxide particles. The encapsulation of iron oxide could shield them from interacting with vascular components, as well as the sustain release of free iron ions, which may ultimately reduce the side effects . Furthermore, the polymeric nanocarriers can conjugate tumor-targeting molecules for providing brain glioma-specific detection. However, the cytotoxicity, non-biodegradable, and limitation in functional possibility and flexibility restrict the clinical application of polymers as MRI contrast agent [13, 14]. Moreover, previous studies have focused on enhancing the contrast capabilities of the polymeric nanocarriers with incorporation of iron oxide, and their active tumor-targeting potential has not been well explored.
We have successfully synthesized a novel amphiphilic poly(aminoethyl ethylene phosphate)/poly(L-lactide) (PAEEP-PLLA) copolymer by ring-opening polymerization reaction, which contains hydrophobic PLLA and hydrophilic PAEEP segments with good biocompatibility and biodegradability . The PAEEP segment containing a large number of amino groups can be modified with tumor-targeting molecules, such as lactoferrin (Lf), an effective targeting ligand for gliomas. In this study, oleylamine-coated Fe3O4 magnetic nanoparticles (OAM-MNPs) were synthesized and encapsulated in PAEEP-PLLA nanoparticles (M-PAEEP-PLLA-NPs). M-PAEEP-PLLA-NPs were further conjugated with Lf (Lf-M-PAEEP-PLLA-NPs) for glioma tumor targeting. The physiochemical properties, biocompatibility, and the brain glioma targeting MRI effect of Lf-M-PAEEP-PLLA-NPs in vitro and in vivo were studied.
Iron (III) acetylacetonate (Fe(acac)3), oleylamine, and benzyl ether were purchased from Acros Organics (Geel, Belgium). Lf from bovine colostrum and fluorescent aminoluciferin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Poly(vinyl alcohol) (PVA-217, 1700 of polymerization degree, 88.5 % of hydrolysis degree) was obtained from Kuraray (Tokyo, Japan). Chloroform was purchased from Sinopharm (Shanghai, China). α-Malemidyl-ω-N-hydroxysuccinimidylpoly(ethylene glycol) (NHS-PEG-MAL, MW3400) was obtained from Jenkem Technology (Beijing, China). Male Wistar rats (250–300 g) were supplied by Hubei Center for Disease Control and Prevention (Animal Qualification Certificate No. 4200060003926). All other reagents were of analytical grade.
Synthesis of OAM-MNPs
OAM-MNPs were synthesized using the thermal decomposition method as described previously . Briefly, 3 mmol Fe(acac)3 was dispersed in a solution of 7.5 mL oleylamine and 7.5 mL benzyl ether. The mixture was dehydrated at 110 °C for 1 h under nitrogen atmosphere with magnetic stirring, then heated to 298 °C at a rate of 15 °C/5min, and aged at 298 °C for 1 h. The solution was cooled to room temperature, added with ethanol for precipitation and centrifuged at 7000 rpm for 10 min. The precipitates containing OAM-MNPs were harvested, washed with ethanol three times, and dried at 60 °C overnight.
Preparation of Lf-M-PAEEP-PLLA-NPs
M-PAEEP-PLLA-NPs were prepared by ultrasonic emulsification and solvent evaporation method. PAEEP-PLLA (100 mg) and OAM-MNPs (100 mg) were dissolved in 1 mL chloroform. The mixture was drop-wise added into 10 mL 0.3 % PVA solution with 2-min sonication at 100 W. The formed oil/water (O/W) emulsion was stirred at room temperature overnight and centrifuged at 7000 rpm for 10 min. The precipitates were resuspended in normal saline solution and stored at 4 °C.
The conjugation of M-PAEEP-PLLA-NPs with Lf was performed through a PEG spacer as described in our previous work . First, sulfhydryl groups were linked to Lf molecules using Traut’s reagent; the product obtained was Lf sulfhydryl. Second, the M-PAEEP-PLLA-NPs was incubated with α-malemidyl-ω-N-hydroxysuccinimidylpoly(ethylene glycol). Finally, glycine solution was added to quench the reaction and the obtained mixture was incubated with Lf sulfhydryl. The Lf-M-PAEEP-PLLA-NPs were purified by centrifugation. The formation of OAM-MNPs, M-PAEEP-PLLA-NPs, and Lf-M-PAEEP-PLLA-NPs were monitored by Fourier transform infrared spectroscopy (FTIR, VERTEX 70, Germany).
Characterization of OAM-MNPs and Lf-M-PAEEP-PLLA-NPs
The surface morphology of OAM-MNPs and Lf-M-PAEEP-PLLA-NPs were examined using transmission electron microscopy (TEM, JEM-2010, Japan) at an accelerating voltage of 100 kV. The hydrodynamic size and polydispersity index (PDI) were measured by photon correlation spectroscopy (PCS, Nano-ZS90 zetasizer, UK) at 25 °C using He-Ne laser of 633 nm.
Thermogravimetric analyses (TGA) of OAM-MNPs and Lf-M-PAEEP-PLLA-NPs were obtained with a thermogravimetric analyzer (EXSTAR TG/DTA 6000 Series, Japan); 10 mg sample was heated at 100 °C for 30 min to remove all free water. The pyrolysis property was examined from 100 to 960° C heated at a constant rate of 15 °C/min. Nitrogen was used as the purge gas at a 50-mL/min flow rate for the thermal stability.
The crystal structures of OAM-MNPs and Lf-M-PAEEP-PLLA-NPs were studied by X-ray diffraction (XRD) on a diffractometer (X′Pert Powder, Netherlands) with Cu Kα radiation(40 kV, 100 mA, and λ = 0.1541 nm). Patterns were recorded in the continuous scanning mode from 10 to 90 °C with a step size of 0.0131° and a step time of 9.945 s.
The magnetic properties of OAM-MNPs and Lf-M-PAEEP-PLLA-NPs were characterized by the vibrating sample magnetometer (VSM, Model 7404, USA) using maximum fields of 15,000 Oe at room temperature.
Rat C6 glioma (C6 cells) and human normal liver cell line (HL-7702) were purchased from Shanghai Institute of Life Science Cell Culture Center (Shanghai, China). C6 cells and HL-7702 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10 % fetal calf serum (FCS) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), penicillin (100 IU/mL), and streptomycin (100 mg/mL), in humidified air containing 5 % CO2 at 37 °C. The in vitro cytotoxicity was evaluated by MTT assay. Cells were seeded at 5 × 103 cells/well in 96-well plates and incubated overnight; 0.1 mL of DMEM (control), M-PAEEP-PLLA-NPs, or Lf-M-PAEEP-PLLA-NPs solution (12.5, 25, 50, 75, 100 μg/mL) was added and incubated for 24 or 48 h. Then, 20 μL of MTT solution (5 mg/mL) was added to each well and the plate was incubated at 37 °C for 4 h. After incubation, the absorbance was measured at 492 nm using a microplate reader (318C-Microplate Reader, China).
Effect of Lf on Cellular Uptake
C6 cells and ECV304 cells were used to investigate the cellular uptake of Lf-M-PAEEP-PLLA-NPs and M-PAEEP-PLLA-NPs. Cells were seeded in 12-well plates at a density of 150,000 cells/well in DMEM and incubated for 24 h. Then, Lf-M-PAEEP-PLLA-NPs or M-PAEEP-PLLA-NPs was added in cell culture medium at concentrations of 5, 10, 15, and 20 μg/mL. After 2-h incubation, the medium was removed and cells were washed with PBS for three times. To remove cell debris, samples were centrifuged at 2000 rpm for 10 min, followed by resuspending in double distilled water. The supernatant was analyzed by flow cytometry (Beckman Coulter, France). Fluorescent aminoluciferin was labeled onto the nanoparticles for the flow cytometric cell uptake study.
In Vitro MR Imaging
The MR T 2 relaxivity of the nanoparticles was determined using 3.0-T whole-body MR scanner (MAGNETOM Trio, A Tim System 3T, Siemens, Germany) in combination with an eight-channel wrist joint coil. Lf-M-PAEEP-PLLA-NPs and M-PAEEP-PLLA-NPs were diluted in 1 % agarose at an iron concentration range of 0–5 μg/mL and then transferred to a 96-well plate. T 2-weighted images were acquired using a spin echo sequence. The parameters are field of view = 120 mm, base resolution = 384 × 384, slice thickness = 1.5 mm, multiple echo times = 20, 40, 60, 80, 100, 120, and 140 ms, repetition time = 2000 ms, and scan time = 13 min. T 2 relaxation rates were plotted against iron concentrations in the particle dilutions. The relaxivity was determined by a linear fit.
In Vivo MR Imaging
All animal studies were approved by the Animal Experimentation Ethics Committee of Huazhong University of Science and Technology and carried out in compliance with guidelines approved by the Science and Technology Department of Hubei Province.
Nine rats were anesthetized with 10 % chloral hydrate (i.p., 4 mL/kg) and placed in a stereotactic frame. A burrhole was drilled through the skull 1 mm posterior and 3 mm lateral to the bregma. A 25-μL microinjector was used to inject 10-μL (1 × 106) C6 cells. The injection was done slowly over 10 min and stayed 10 min, and the needle was withdrawn after another 10 min. The skin was closed with nonmagnetic sutures. MRI was performed after tumor growth for 2 weeks as described in previous reports [17, 18, 19].
SIpre was measured before injection and SIpost was measured at each follow-up time after injection (2, 4, 6, 8, 12, 24, and 48 h).
After MRI, all rats were anaesthetized and perfused with PBS solution followed by 4 % paraformaldehyde. Brain glioma were removed and fixed in 4 % paraformaldehyde for 24 h, embedded in paraffin, slided at 5 μm, and stained with hematoxylin and eosin (H&E) and Prussian blue.
All data were expressed as mean ± SD. Unpaired student’s t test was used to examine the differences among various groups. P values less than 0.05 were considered statistically significant. All statistical analyses were performed using SPSS, Version 19.
Results and Discussion
Preparation and Characterization of Lf-M-PAEEP-PLLA-NPs
In this study, OAM-MNPs were encapsulated in PAEEP-PLLA copolymer nanoparticles. PAEEP segment is highly hydrophilic and PLLA segment is hydrophobic, and both PAEEP and PLLA are biodegradable . So the obtained PAEEP-PLLA block copolymer is amphipathic and biodegradable with the combined appealing features of PAEEP and PLLA. Hydrophilic polyphosphate chains can effectively prevent the nanoparticles from being cleared by RES in the blood. Moreover, PAEEP segment contains a large number of amino groups, which can be readily modified with tumor-targeting molecules. Lf, a mammalian cationic iron-binding glycoprotein belonging to the transferrin family, contains a polypeptide chain with 680–690 amino acids. In our recent work, Lf has been proved as an effective targeting ligand for brain gliomas [17, 18].
In Vitro Studies
The biocompatibility of M-PAEEP-PLLA-NPs and Lf-M-PAEEP-PLLA-NPs was evaluated by the cytotoxicity assay of C6 cells and HL-7702 cells. A viability of over 90 % in the two cell lines was maintained by increasing the M-PAEEP-PLLA-NPs and Lf-M-PAEEP-PLLA-NPs concentration from 12.5 to 100 μg/mL. The results indicated that M-PAEEP-PLLA-NPs and Lf-M-PAEEP-PLLA-NPs had no obvious cytotoxicity to C6 cells and HL-7702 cells within the concentration range used during the in vivo imaging. Thus, the cytotoxicity studies suggested that M-PAEEP-PLLA-NPs and Lf-M-PAEEP-PLLA-NPs had good biocompatibility and suitability for biomedical applications.
In Vivo MR Imaging
The results indicated strong, long-lasting, tumor-targeting, and contrast-enhanced MRI ability of Lf-M-PAEEP-PLLA-NPs owing to the selectively accumulation in brain glioma tissue. Moreover, there were studies that showed that Lf mediates the blood–brain barrier (BBB) transportation by the reorganization of Lf receptor (LfR) on the BBB [25, 26]. With the unidirectional receptor-mediated transcytosis, polymer nanocarriers conjugated with Lf could cross the BBB and enter into the brain. So we can conclude that Lf could strengthen the ability of the Lf-M-PAEEP-PLLA-NPs through the BBB and binding to brain glioma cells with LfR. These suggested that Lf-M-PAEEP-PLLA-NPs could be an optimal contrast agent for brain glioma targeting MR imaging.
In this study, oleylamine-coated superparamagnetic Fe3O4 nanoparticles were successfully synthesized and encapsulated in PAEEP-PLLA nanoparticles with ultrasonic emulsification and solvent evaporation method. Lf, an effective targeting ligand for brain glioma cells, was conjugated to the Lf-M-PAEEP-PLLA-NPs. We observed strong, long-lasting, tumor-targeting, and contrast-enhanced MR imaging ability of Lf-M-PAEEP-PLLA-NPs by in vitro and in vivo MRI studies. Our results suggest that Lf-M-PAEEP-PLLA-NPs represent a promising nano-sized MRI contrast agent for brain glioma targeting.
The authors have given their consent for the case reports to be published. Written informed consent was obtained from the author for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
This work was financially supported by National Science Foundation of China (NSFC, 31170960, 31470968and 81372369) and National High Technology Research and Development Program of China (863 Program, 2012AA022704).
The authors report no conflicts of interest in this work.
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