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Genome Biology

, 3:spotlight-20021113-01 | Cite as

Chips for viral pathogens

  • Jonathan B Weitzman
Research news

Keywords

Tract Infection Respiratory Tract Respiratory Tract Infection Oligonucleotide Primer Lavage Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Easy and reliable viral detection is important for clinical diagnosis, agricultural analysis and monitoring the threat of bioterrorism. In the Early Edition of the Proceedings of the National Academy of Sciences, Wang et al. describe a genomic strategy for efficient viral detection using microarrays. In an attempt to overcome the limitations imposed by conventional PCR-based detection, they designed a long oligonucleotide (70-mer) DNA microarray that can simultaneously detect hundreds of viruses. A random PCR amplification step avoided the need for sequence-specific or degenerate oligonucleotide primers. The microarray was tested with nasal lavage fluid from individuals with respiratory tract infection to demonstrate its efficacy in a clinical setting.

References

  1. 1.
    Proceedings of the National Academy of Sciences , [http://www.pnas.org]
  2. 2.
    Microarray-based detection and genotyping of viral pathogens, [http://www.pnas.org/cgi/doi/10.1073/pnas.242579699]
  3. 3.
    Microarray Based Detection and Genotyping of Viral Pathogens supplemental data, [http://derisilab.ucsf.edu/virochip]

Copyright information

© BioMed Central Ltd 2002

Authors and Affiliations

  • Jonathan B Weitzman

There are no affiliations available

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