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Genome Biology

, 3:spotlight-20020709-01 | Cite as

RNAi in adult mice

  • Jonathan B Weitzman
Research news

Keywords

Adult Mouse Firefly Luciferase Luciferase Expression Transfection Method Firefly Luciferase Reporter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Small interfering RNAs (siRNAs) have shown great potential as gene silencing reagents in a wide range of experimental systems. In the July 4 Nature, Anton McCaffrey and colleagues at the Stanford University School of Medicine report the use of siRNAs to inhibit transgene expression in adult mice and to target hepatitis C virus sequences in vivo (Nature 2002, 418:38-39). They used a modified hydrodynamic transfection method to deliver naked siRNAs to the liver of adult mice. They then monitored expression of a firefly luciferase reporter transgene using whole-body imaging techniques. McCaffrey et al. report specific siRNA-mediated inhibition (around 80%) of luciferase expression. They also found that small-hairpin RNAs, transcribed in vivofrom DNA templates, could induce gene silencing in mice. When siRNA was directed against the NS5B (non-structural protein 5B, viral polymerase-encoding region) it was also effective, suggesting that RNAi-based strategies may be used therapeutically to target human pathogens.

References

  1. 1.
    Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.Google Scholar
  2. 2.
  3. 3.
    Stanford University School of Medicine , [http://www.med.stanford.edu/]

Copyright information

© BioMed Central Ltd 2002

Authors and Affiliations

  • Jonathan B Weitzman

There are no affiliations available

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