Nuclear transcription factors in mammalian mitochondria
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Nuclear transcription factors have been detected in mammalian mitochondria and may directly regulate mitochondrial gene expression. Emerging genomics techniques may overcome outstanding challenges in this field.
KeywordsMitochondrial Genome Mitochondrial Biogenesis Nuclear Transcription Factor Mitochondrial Gene Expression Mitochondrial Transcription Factor
Mammalian cells store genetic information in two cellular compartments: the nucleus and the mitochondria. Mitochondrial DNA is packaged, handled and inherited independently of the nuclear genome, and far less is known about the regulation of mitochondrial gene expression compared with that of nuclear genes. As the singular site of the generation of adenosine triphosphate (ATP) by oxidative phosphorylation in the eukaryotic cell, the regulation of mitochondrial functions are complex and must be tightly regulated to respond to cellular metabolic requirements [1, 2]. The majority of proteins present in mitochondria are encoded and transcribed in the nucleus , but the mitochondrial genome encodes a handful of proteins crucial for the generation of ATP (Figure 1). These proteins are transcribed and translated in the mitochondrial matrix and do not enter the cytoplasm [4, 5]. Because both the nuclear and the mitochondrial genomes contribute to the mitochondrial proteome, their regulatory coordination is critical to cell survival and energy homeostasis . This coordination is complicated by the distinct packaging and environment of the two genomes (Box 1).
Box 1 Mitochondria and mitochondrial gene regulation
The mitochondrion is the single cellular site of ATP generation via aerobic respiration, and metabolites such as dietary lipids and pyruvate, the metabolic product of glycolysis, are actively transported into mitochondria . As the tricarboxylic acid cycle progresses within the mitochondrial matrix, a series of electron-transfer reactions, known collectively as the electron-transport chain, proceeds between large multiprotein complexes and small electron carriers within the inner membrane and matrix . The resulting electrochemical gradient generates bioavailable ATP via a rotating inner-membrane ATPase, which couples proton flow down a proton gradient to the catalysis of the phosphorylation of ADP to ATP using inorganic phosphate .
Nuclear transcription factors were first identified as acting in mitochondria 15 years ago, and these early reports included the glucocorticoid receptor , the tumor suppressor p53  and the mitochondrial receptor for the thyroid hormone tri-iodothyronine (T3), named p43 . The crucial demonstration that mitochondrial gene expression can be regulated without a change in nuclear gene expression came a few years later, using biochemically isolated mitochondria. The observation that thyroid hormone treatment of isolated mitochondria altered mitochondrial gene expression despite the absence of a nucleus indicated that mitochondrial gene expression was not only regulated indirectly, via the nucleus. It also suggested that a T3 receptor might be acting directly within the mitochondria as a regulator of transcription from the mitochondrial genome.
Mitochondrial dysfunction is known to contribute to aging  and to diseases, including cancer , diabetes  and obesity , but despite the boom in transcription factor biology facilitated by emerging technologies such as chromatin immunoprecipitation (ChIP), the mitochondrial role of nuclear transcription factors is understudied. This is in part due to technical challenges of disambiguating indirect regulation from direct regulation when both are mediated by the same transcription factor or signaling pathway. In this review we discuss the methods used to dissect the functions of nuclear and mitochondrial transcription factors, and discuss five well-characterized examples of nuclear-encoded gene regulatory proteins that act in mitochondria.
Techniques for distinguishing nuclear and mitochondrial roles of mammalian transcription factors
Techniques used to investigate nuclear transcription factors in mitochondria
Experimental question: Is the factor localized to mitochondria?
Centrifugation separates the mitochondrial, nuclear and cytoplasmic fractions of a cell sample. Immunoblotting for known mitochondrial, nuclear and cytoplasmic proteins assesses the efficiency of the fractionation process. Further immunoblotting establishes presence of factor of interest in mitochondrial fraction.
Labeling of intact cell preparations with specific antibodies conjugated to a heavy metal, such as gold. Transmission electron microscopy is used to visually analyze co-localization of the gold with the distinctly identifiable mitochondria.
Mitochondrial import analysis
Observing the uptake of an in vitro translated radiolabeled protein of interest into isolated intact mitochondria, in the absence of a nucleus or any external stimulation.
Inference of mitochondrial localization for a protein by characterization of a physical interaction with a known mitochondrial protein.
Experimental question: Is the putative mitochondrial role independent from the factor's nuclear role?
In organello systems
Isolation of intact mitochondria and observation of their response to stimulation of the factor of interest (for example, by addition of a hormone ligand).
Overexpression of a mitochondrion-specific isoform of a transcription factor or fusion of a transcription factor with a constitutive mitochondrial localization signal. Altered transcription from the mitochondrial genome in the absence of altered nuclear target expression suggests direct regulation of mitochondrial gene expression by that factor.
Experimental question: Does the factor bind to the mitochondrial DNA and/or regulate mitochondrial gene expression?
Chromatin immunoprecipitation (ChIP)
Assay of specific protein-DNA interactions by the crosslinking of proteins to DNA followed by antibody-based enrichment of a protein of interest. The DNA bound to that factor can then be assayed site-specifically by quantitative PCR, or on a genome-wide scale by microarray analysis or high-throughput sequencing. Used to detect direct binding of a factor to mtDNA.
Assay of protein-DNA interactions nonspecifically by crosslinking protein to DNA followed by DNase digestion or dimethylsulfate treatment. Protein-bound DNA sequences are protected from digestion or methylation. Regions of mtDNA are then assayed for a change in protection pattern following stimulation of a specific protein or pathway.
Electro-mobility shift assay (EMSA)
Detects the capacity of a pool of proteins (for example, a mitochondrial extract) to bind to a short sequence of synthesized DNA, causing it to run slower (and thus 'shift' higher) than non-complexed DNA on a non-denaturing polyacrylamide gel. The addition of antibodies against the protein of interest gives specificity. Antibodies can be applied before mixing extract with DNA, resulting in loss of 'shift', or afterwards resulting in increased 'shift'.
Transcriptional reporter assays
Use of nuclear transcription constructs containing a putative regulatory sequence derived from mtDNA, upstream of a nuclear promoter and a reporter gene such as luciferase. Changed expression of the construct following stimulation of the transcription factor of interest shows that these mitochondrial sequences can act as regulatory elements when placed in a nuclear context.
Proteomics studies over the past decade have attempted to characterize the complete mammalian mitochondrial proteome [1, 2, 18]. While these studies have revealed the responsiveness of mitochondria to cell signaling and the tissue specificity of mitochondrial function, they have rarely identified direct mitochondrial transcriptional regulators. DNA-damage repair proteins and other DNA-binding proteins seem difficult to detect using mass spectrometry because of their low relative abundance . Subtractive proteomics - the differential detection of proteins in a mitochondrial sample relative to a cytoplasmic/nuclear sample - can increase the sensitivity of a mass spectrometric analysis, but at the cost of not detecting proteins with a role in multiple organelles . Both the proteomics and genomics tools used in mitochondrial studies are improving rapidly, and as a result the number of nuclear transcription factors shown to play a direct role in mitochondrial regulation is likely to increase.
Nuclear transcription factors as direct regulators of mitochondrial gene expression
The nuclear transcription factors best characterized as direct regulators of mitochondrial gene expression in mammals are the T3 receptor p43, CREB, the tumor suppressor p53, signal transducer and activator of transcription 3 (Stat3) and the estrogen receptor. p43 and CREB are transcription factors that can bind mitochondrial DNA to regulate gene expression. p53, Stat3, and, potentially, the estrogen receptor are thought to act as co-regulators, affecting mitochondrial gene expression through protein-protein interactions.
T3 also regulates mitochondrial function directly via two pathways: the regulation of nucleotide transport across the inner membrane via a T3-binding adenine nucleotide transporter (AdNT) [25, 26]; and control of mitochondrial transcription via the mitochondrially localized T3Rα1 isoform known as p43 (Figure 3; reviewed in [27, 28], see also ). Most proteins are imported into mitochondria in an ATP-dependent manner via the protein translocator channel TOM, which recognizes an amino-terminal mitochondrial localization signal that is then cleaved during import. p43, however, is imported into rat liver mitochondria via a different pathway, previously shown for the yeast mitochondrial transcription factor MTF1 , which is independent of both TOM and mitochondrial ATP levels, and does not result in cleavage of the imported protein.
One potential mechanism by which transcription factors could be accurately sorted among different cellular compartments is revealed when considering the T3 receptors. It was noticed that a protein construct mimicking a T3Rβ isoform with a truncated amino terminus (which is the form present in most non-mammalian vertebrates) is specifically imported into isolated rat mitochondria, suggesting a role for the amino-terminal truncation in mitochondrial import . p43 is itself a truncated form of the full-length nuclear transcription factor T3Rα and is translated from an alternative start site in the T3Rα mRNA [15, 29]. The expression of p43 is, however, regulated independently from the full-length T3Rα and it shows a distinct tissue-specific pattern of expression .
T3 receptors associate with nuclear DNA in a sequence-specific manner via T3-response elements (T3REs), DNA motifs first recognized in the promoters of T3-responsive genes. Multiple T3REs have been identified in the mouse mitochondrial genome that confer responsiveness to thyroid hormone in nuclear reporter assays, and p43 binds to these sequences in vitro in EMSAs. These techniques do not, however, address the question of whether p43 binds mitochondrial DNA in vivo under physiological conditions. This was partly addressed in a series of studies utilizing induced-hypothyroid rats, in which physiological T3 levels were found to regulate mitochondrial gene expression directly in vivo. Mitochondria isolated from the livers of these rats showed that changes in physiological thyroid hormone levels altered the relative levels of mitochondrial mRNA and rRNA, which correlated with altered protein occupation of the mitochondrial D-loop as determined by DNA footprinting . The independence of this direct mitochondrial role for T3 from the well-characterized indirect role was shown when isolated mitochondria from hypothyroid rats were treated with T3 and the mitochondrial mRNA:rRNA ratio and the pattern of DNA footprinting returned to that of normal rats . This indicated that T3 regulates mitochondria directly and suggested that this pathway may involve a mitochondrial T3 receptor with similar binding preferences to the nuclear form.
The role of p43 in T3-mediated regulation of mitochondrial transcription was confirmed using the same in organello system from induced-hypothyroid rats to show that the addition of p43 (translated in vitro to avoid possible contamination by cellular components) stimulated mitochondrial gene transcription in the presence of T3 , whereas T3 treatment in the absence of p43 did not stimulate mitochondrial gene expression . In validation of the mitochondrial role of p43 in vivo, mice overexpressing p43 under the control of a muscle-specific promoter exhibited increased mitochondrial gene expression and mitochondrial biogenesis in muscle, and had increased oxidative metabolism, with body temperature 0.8°C higher than control mice .
The direct regulation of mitochondrial transcription by T3 is complex and highly tissue specific. In organello studies that demonstrate the responsiveness of liver mitochondrial transcription to T3 also demonstrate that mitochondria from the heart are not regulated in this manner. Rather, T3 regulation of mitochondria from the hearts of the induced-hypothyroid rats is indirect - via the nucleus - and primarily at the level of regulating mitochondrial DNA copy number . This complexity is likely to be shared by other transcription factors with a direct mitochondrial activity, and it may explain why early work did not detect direct binding of a protein to the proposed mitochondrial T3REs  despite the requirement of a DNA-binding domain in p43 for the observed mitochondrial function . Regardless of this outstanding debate over the location of p43 binding to mitochondrial DNA, evidence is overwhelming that p43 is localized to the mitochondria in rat liver, where it binds to the mitochondrial genome and regulates mitochondrial transcription. A great deal remains to be studied regarding p43 in mitochondria - for example, it is not clear whether this regulatory pathway is conserved within mammals, or in which other tissues it is utilized. Nevertheless, the studies on thyroid hormone and thyroid hormone receptor were the first direct illustration that mitochondrial gene expression is regulated independently from nuclear gene expression and introduced a key model system for the study of nuclear transcription factors in mitochondria.
Cyclic-AMP response element binding protein (CREB)
The transcription factor CREB regulates nuclear gene expression in response to a diverse range of stimuli [33, 34]. CREB is activated by phosphorylation, either by the cyclic-AMP responsive protein kinase A (PKA) or by other kinases, including mitogen-activated protein kinases (MAPKs) and Ca2+/calmodulin-dependent kinases (CaMKs) . A self-contained CREB pathway exists in mitochondria, which involves PKA , cyclic AMP  and CREB . On stimulation, this pathway induces binding of phosphorylated CREB to cyclic-AMP response elements (CREs) in the mitochondrial DNA D-loop and regulation of mitochondrial gene expression [39, 40] (Figure 1).
CREB was first localized to rat brain mitochondria by subcellular fractionation followed by immunoelectron microscopy . Despite not having a classical mitochondrial localization signal, the transport of labeled CREB into isolated rat liver mitochondria depends on the mitochondrial translocator TOM, the import route for most proteins into mitochondria . The mitochondrial pool of CREB can co-immunoprecipitate with the chaperone protein mtHSP70 , suggesting a mechanism of targeting to the mitochondria that is dependent on chaperone proteins rather than on a mitochondrial localization signal, as has been shown for p53 . Once in mitochondria, CREB is regulated by phosphorylation in response to the same stimuli as in the nucleus, and in vitro can bind oligonucleotides bearing the consensus CRE sequence . Binding of CREB to the mitochondrial D-loop (Figure 1) has been detected in vivo using ChIP  and DNase footprinting, and is dependent on mitochondrial PKA activity [40, 41]. Unlike p43, mitochondrial localization of CREB has been identified in multiple mammalian species and tissues [36, 38, 39].
An overexpression construct that selectively increases levels of CREB in mitochondria was used to distinguish CREB nuclear and mitochondrial regulatory roles in primary cultured neurons from rat brain . These increases in mitochondrial CREB perturbed mitochondrial gene expression without altering the expression levels of CREB's nuclear target, c-fos . The mRNAs of mitochondrially encoded NADH dehydrogenase subunits 2, 4 and 5 (Figure 1) were specifically upregulated; conversely, these mRNAs were downregulated on treatment with a dominant-negative form of CREB in the mitochondria .
Tumor suppressor protein p53
The tumor suppressor protein p53 is a well-known example of a nuclear transcription factor with a role in mitochondria . First identified by its transcriptional regulatory function , p53 also has non-transcriptional functions, and has been implicated in apoptosis , senescence , autophagy , DNA-damage repair and cell-cycle arrest .
In mitochondria, p53 directly regulates apoptosis via protein-protein interactions at the outer membrane, and this function has been reviewed thoroughly elsewhere [48, 49]. However, there is considerable evidence for a second mitochondrial role for p53, in mitochondrial DNA maintenance and in mitochondrial DNA-damage repair. Co-immunoprecipitation of p53 with the mitochondrion-specific transcription and mitochondrial DNA packaging factor Tfam suggests that p53 may regulate DNA-damage repair in mitochondria , as it does in the nucleus [51, 52]. In KB human epidermoid cancer cells and in HCT116 adenocarcinoma cells, p53 physically interacts with Tfam, with the effect of enhancing the binding of Tfam to cisplatin-damaged DNA at the expense of oxidized DNA, in a reversal of Tfam's normal binding pattern .
p53 also seems to play a role in mitochondrial base-excision repair. In a nucleus-free in vitro system derived from the mitochondria of mouse liver, p53 can stimulate the gap-filling function of the mitochondrial DNA polymerase mtPOLγ . A physical interaction between p53 and mtPOLγ in vivo has been detected in HCT116 cells , where p53 enhances the replication function of mtPOLγ and interacts with the mitochondrial genome. The observed binding of p53 to the mitochondrial genome was stimulated by, but not dependent on, DNA damage, suggesting that the role of p53 at the mitochondrial DNA may not be confined to the DNA-damage response. Furthermore, in studies comparing mitochondria from p53-deficient cell lines with those from isogenic p53-positive lines, p53 appears to provide an endogenous proofreading function for mtPOLγ during mitochondrial DNA replication .
Despite clear localization of p53 to the mitochondrial matrix and a number of direct associations with mitochondrial DNA, evidence that p53 can bind sequence-specifically to regulate expression of mitochondrially encoded genes remains elusive. Sequences from the mouse mitochondrial genome that resemble the nuclear binding motif of p53 confer p53 responsiveness in nuclear reporter assays , but there is no evidence that p53 regulates transcription from the mitochondrial genome. Regardless of whether p53 directly regulates mitochondrial transcription, it plays important mitochondrial roles in apoptosis, DNA integrity and response to stress.
Signal transducer and activator of transcription 3 (Stat3)
Stat3 was first detected in mitochondria as a result of its functional association with GRIM-19, a subunit of the respiratory electron-transport chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain [57, 58]. In the nucleus, Stat3 mediates the transcriptional response to growth factors such as interleukin-6 and epithelial growth factor . Differences between Stat3 function in the mitochondria and nucleus are exemplified by the fact that the mitochondrial pool of Stat3 mediates oncogenic transformation by the small GTPase H-Ras, a process that is mechanistically distinct from how nuclear Stat3 supports oncogenic transformation by the viral oncogene v-Src . Both Stat3-knockdown cell lines and Stat3-knockout mice show disrupted electron-transport chain function , which suggests that Stat3 directly regulates mitochondrial function via its effects on the electron-transport chain. Engineered Stat3 mutant proteins have shown that the nuclear role and the mitochondrial role can be functionally isolated .
The estrogen receptor
The estrogen receptor was first found to localize to mitochondria of rabbit uterus and ovary in 2001 . This receptor regulates gene expression by binding to estrogen-response elements (EREs) in gene promoters following the binding of the steroid hormone estrogen to the receptor . Nuclear targets include NRF-1, which, as noted earlier, encodes a transcription factor that stimulates mitochondrial biogenesis and is a transcriptional regulator of genes encoding the mitochondrial basal transcription machinery  (Figure 2). The indirect regulation of mitochondrial function by the actions of the estrogen receptor has been reviewed elsewhere [65, 66].
There is evidence that estrogen acts directly in mitochondria by two pathways: one utilizing the receptor and the other independent of it [27, 67, 68]. The presence of the estrogen receptor in mitochondria is well established; both isoforms, ERα and ERβ, localize to mitochondria in diverse cell lines and tissues, yet their functions remain contentious. EMSAs suggest that ERβ may bind directly to the D-loop of the mitochondrial genome in MCF-7 breast cancer cells (Figure 1). This binding was stimulated by treatment of the cells with estrogen and inhibited by treatment with ERβ-specific antibodies . It has not, however, been shown that isolated mitochondria respond to estrogen treatment by altering gene expression in an ERβ-dependent fashion.
Other putative functions for the mitochondrial estrogen receptor have focused on protein-protein interactions identified using a bacterial two-hybrid screen. This screen revealed that ERα can interact stably and reproducibly with the mitochondrial protein 17β-hydroxysteroid dehydrogenase type 10, suggesting a role for mitochondrial ERα in regulating cellular steroid metabolism and response .
Since cancer is in part a metabolic disease , and altered mitochondrial DNA sequence and transcription levels have been observed in both primary tumors and in cancer cell lines [72, 73], a mitochondrial role for the estrogen receptor would be relevant to both estrogen receptor biology and the study of hormone-sensitive breast cancer. As with other nuclear factors, however, the indirect action of the estrogen receptor on mitochondrial gene expression is a confounding factor that complicates investigation. Furthermore, as noted above, estrogen seems to regulate mitochondria directly, even in the absence of its receptor . The disagreement over the role of mitochondrial estrogen receptors could be, in part, due to cell-specific functions. Nevertheless, mitochondria are clearly an important target of estrogen hormone action.
Other transcription factors
Although only a small number of nuclear transcription factors have had a mitochondrial role validated, either in a nucleus-free in organello system or by detection of binding to the mitochondrial genome, there are a number of nuclear transcription factors that have been localized to mitochondria but where the mitochondrial role remains understudied. The glucocorticoid receptor , the heterodimeric transcription factor AP-1 , and the peroxisome proliferator-activated receptor γ (PPARγ)  have all been detected in mammalian mitochondria, and there is some evidence for the glucocorticoid receptor  and AP-1  binding to the mitochondrial genome to potentially regulate gene expression.
The five nuclear transcription factors that have been shown to have distinct mitochondrial roles are all involved in signaling pathways: the estrogen receptor and p43 are nuclear hormone receptors activated by a hormone ligand; CREB is phosphorylated in a cyclic-AMP-dependent manner; Stat3 is stimulated by growth hormone signaling pathways; and transcription of p53 is activated in response to cell stress. The same signals and signaling pathways regulate the mitochondrial pools of these proteins, so the mitochondrial and the nuclear roles appear to be coordinated regulatory responses. Because these proteins are from different families and the characterized mitochondrial functions are varied, it seems that the development of a mitochondrial role for a nuclear transcription factor is likely to be a common evolutionary strategy for coordinating the two genomes.
The mitochondrial genome and proteome are not only regulated indirectly via processes within the nucleus; they are independently responsive to the needs of the cell. The nuclear transcription factors present in mitochondria are involved in oxidative phosphorylation, cellular metabolism and apoptosis, and their mitochondrion-specific roles are a key part of their biology. The mitochondrial roles of nuclear transcription factors are likely to form a core part of their cellular functions, and yet have been explored in detail in only a handful of cases. The application of new genomics and proteomics techniques may substantially revise our understanding of the regulatory interactions that exist between the nuclear and mitochondrial genomes - with implications for transcription factor biology, mitochondrial regulation and diseases such as cancer, diabetes and obesity.
- 1.Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M: Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell. 2003, 115: 629-640. 10.1016/S0092-8674(03)00926-7.PubMedCrossRefGoogle Scholar
- 2.Pagliarini DJ, Calvo SE, Chang B, Sheth SA, Vafai SB, Ong SE, Walford GA, Sugiana C, Boneh A, Chen WK, Hill DE, Vidal M, Evans JG, Thorburn DR, Carr SA, Mootha VK: A mitochondrial protein compendium elucidates Complex I disease biology. Cell. 2008, 134: 112-123. 10.1016/j.cell.2008.06.016.PubMedPubMedCentralCrossRefGoogle Scholar
- 8.Demonacos C, Tsawdaroglou NC, Djordjevic-Markovic R, Papalopoulou M, Galanopoulos V, Papadogeorgaki S, Sekeris CE: Import of the glucocorticoid receptor into rat liver mitochondria in vivo and in vitro. J Steroid Biochem Mol Biol. 1993, 46: 401-413. 10.1016/0960-0760(93)90231-K.PubMedCrossRefGoogle Scholar
- 10.Wrutniak C, Cassar-Malek I, Marchal S, Rascle A, Heusser S, Keller JM, Fléchon J, Dauça M, Samarut J, Ghysdael J, Cabello G: A 43-kDa protein related to c-ErbAα1 is located in the mitochondrial matrix of rat liver. J Biol Chem. 1995, 270: 16347-16354. 10.1074/jbc.270.27.16347.PubMedCrossRefGoogle Scholar
- 15.Casas F, Rochard P, Rodier A, Cassar-Malek I, Marchal-Victorion S, Wiesner RJ, Cabello G, Wrutniak C: A variant form of the nuclear triiodothyronine receptor c-ErbAα1 plays a direct role in regulation of mitochondrial RNA synthesis. Mol Cell Biol. 1999, 19: 7913-7924.PubMedPubMedCentralCrossRefGoogle Scholar
- 17.Pláteník J, Balcar VJ, Yoneda Y, Mioduszewska B, Buchal R, Hynek R, Kilianek L, Kuramoto N, Wilczynski G, Ogita K, Nakamura Y, Kaczmarek L: Apparent presence of Ser133-phosphorylated cyclic AMP response element binding protein (pCREB) in brain mitochondria is due to cross-reactivity of pCREB antibodies with pyruvate dehydrogenase. J Neurochem. 2005, 95: 1446-1460. 10.1111/j.1471-4159.2005.03471.x.PubMedCrossRefGoogle Scholar
- 27.Psarra AMG, Solakidi S, Sekeris CE: The mitochondrion as a primary site of action of steroid and thyroid hormones: Presence and action of steroid and thyroid hormone receptors in mitochondria of animal cells. Mol Cell Endocrinol. 2006, 246: 21-33. 10.1016/j.mce.2005.11.025.PubMedCrossRefGoogle Scholar
- 31.Casas F, Pessemesse L, Grandemange S, Seyer P, Baris O, Gueguen N, Ramonatxo C, Perrin F, Fouret G, Lepourry L, Cabello G, Wrutniak-Cabello C: Overexpression of the mitochondrial T3 receptor induces skeletal muscle atrophy during aging. PLoS ONE. 2009, 4: e5631-10.1371/journal.pone.0005631.PubMedPubMedCentralCrossRefGoogle Scholar
- 40.Lee J, Kim CH, Simon DK, Aminova LR, Andreyev AY, Kushnareva YE, Murphy AN, Lonze BE, Kim KS, Ginty DD, Ferrante RJ, Ryu H, Ratan RR: Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. J Biol Chem. 2005, 280: 40398-40401. 10.1074/jbc.C500140200.PubMedPubMedCentralCrossRefGoogle Scholar
- 46.Tasdemir E, Maiuri MC, Galluzzi L, Vitale I, Djavaheri-Mergny M, D'Amelio M, Criollo A, Morselli E, Zhu C, Harper F, Nannmark U, Samara C, Pinton P, Vicencio JM, Carnuccio R, Moll UM, Madeo F, Paterlini-Brechot P, Rizzuto R, Szabadkai G, Pierron G, Blomgren K, Tavernarakis N, Codogno P, Cecconi F, Kroemer G: Regulation of autophagy by cytoplasmic p53. Nat Cell Biol. 2008, 10: 676-687. 10.1038/ncb1730.PubMedPubMedCentralCrossRefGoogle Scholar
- 61.Wegrzyn J, Potla R, Chwae YJ, Sepuri NB, Zhang Q, Koeck T, Derecka M, Szczepanek K, Szelag M, Gornicka A, Moh A, Moghaddas S, Chen Q, Bobbili S, Cichy J, Dulak J, Baker DP, Wolfman A, Stuehr D, Hassan MO, Fu XY, Avadhani N, Drake JI, Fawcett P, Lesnefsky EJ, Larner AC: Function of mitochondrial Stat3 in cellular respiration. Science. 2009, 323: 793-797. 10.1126/science.1164551.PubMedPubMedCentralCrossRefGoogle Scholar
- 63.Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M: Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006, 38: 1289-1297. 10.1038/ng1901.PubMedCrossRefGoogle Scholar
- 66.Chen JQ, Cammarata PR, Baines CP, Yager JD: Regulation of mitochondrial respiratory chain biogenesis by estrogens/estrogen receptors and physiological, pathological and pharmacological implications. Biochim Biophys Acta. 2009, 1793: 1540-1570. 10.1016/j.bbamcr.2009.06.001.PubMedPubMedCentralCrossRefGoogle Scholar
- 67.Bopassa JC, Eghbali M, Toro L, Stefani E: A novel estrogen receptor GPER inhibits mitochondrial permeability transition pore opening and protects the heart against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2010, 298: H16-H23. 10.1152/ajpheart.00588.2009.PubMedPubMedCentralCrossRefGoogle Scholar
- 72.Parrella P, Xiao Y, Fliss M, Sanchez-Cespedes M, Mazarelli P, Rinaldi M, Nicol T, Gabrielson E, Cuomo C, Cohen D, Pandit S, Spencer M, Rabitti C, Fazio VM, Sidransky D: Detection of mitochondrial DNA mutations in primary breast cancer and fine-needle aspirates. Cancer Res. 2001, 61: 7623-PubMedGoogle Scholar
- 76.Casas F, Domenjoud L, Rochard P, Hatier R, Rodier A, Daury L, Bianchi A, Kremarik-Bouillaud P, Becuwe P, Keller J, Schohn H, Wrutniak-Cabello C, Cabello G, Dauça M: A 45 kDa protein related to PPARγ2, induced by peroxisome proliferators, is located in the mitochondrial matrix. FEBS Lett. 2000, 478: 4-8. 10.1016/S0014-5793(00)01814-7.PubMedCrossRefGoogle Scholar
- 77.Demonacos C, Djordjevic-Markovic R, Tsawdaroglou N, Sekeris CE: The mitochondrion as a primary site of action of glucocorticoids: the interaction of the glucocorticoid receptor with mitochondrial DNA sequences showing partial similarity to the nuclear glucocorticoid responsive elements. J Steroid Biochem Mol Biol. 1995, 55: 43-55. 10.1016/0960-0760(95)00159-W.PubMedCrossRefGoogle Scholar
- 79.Maughan R: Carbohydrate metabolism. Surgery. 2009, 27: 6-10.Google Scholar
- 88.Cotney J, McKay SE, Shadel GS: Elucidation of separate, but collaborative functions of the rRNA methyltransferase-related human mitochondrial transcription factors B1 and B2 in mitochondrial biogenesis reveals new insight into maternally inherited deafness. Hum Mol Genet. 2009, 18: 2670-2682. 10.1093/hmg/ddp208.PubMedPubMedCentralCrossRefGoogle Scholar
- 91.Casas F, Pessemesse L, Grandemange S, Seyer P, Gueguen N, Baris O, Lepourry L, Cabello G, Wrutniak-Cabello C: Overexpression of the mitochondrial T3 receptor p43 induces a shift in skeletal muscle fiber types. PLoS ONE. 2008, 3: e2501-10.1371/journal.pone.0002501.PubMedPubMedCentralCrossRefGoogle Scholar