Strain-specific and pathogen-specific physiologic and genomic differences in murine inflammatory cardiac dysfunction
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KeywordsVascular Endothelial Growth Factor Isoflurane Comparative Genomic Analysis Isoflurane Anesthesia Volume Relationship
Comparing genomic changes in mice strains demonstrating physiologic differences with pathologic insults is a novel approach to elucidate potential mechanisms. Our hypothesis was that murine strains exhibit different cardiac/genomic responses to specific pathogens.
The end-systolic pressure–volume relationship (ESPVR) and end-diastolic pressure–volume relationship (EDPVR) cardiac performance was compared in B6, C57 and FVB mice (pressure–volume loops, Millar catheter; isoflurane anesthesia) 4 hours after zymosan (ZYM) or endotoxin (LPS) intraperitoneally. Gene expression profiles unique to mouse strain/baseline/treatment were created using two-way ANOVA/two-fold filtering.
Comparative genomic analyses provide new insights into septic cardiac pathophysiology.
Supported in part by the National Heart, Lung and Blood Institute (Patterson), and the Intensive Care Society (Ackland).
This article is published under license to BioMed Central Ltd.