Advertisement

Critical Care

, 13:P326 | Cite as

Effects of methylprednisolone on sepsis-induced blood–brain barrier permeability and reflex responsiveness in rats

  • F Esen
  • T Erdem
  • A Ogan
  • E Senturk
  • P Ergin Özcan
  • M Kaya
  • N Cakar
  • L Telci
Poster presentation
  • 918 Downloads

Keywords

Methylprednisolone Neurological Function Permeability Change Evans Blue Cecal Ligation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Introduction

The purpose of this study was to assess neurological function and examine blood–brain barrier (BBB) permeability changes in septic encephalopathy after cecal ligation and perforation (CLP). The protective effect of high-dose methylprednisolone administration on BBB derangement and neurological dysfunction was also investigated.

Methods

An experimental prospective randomized study was performed on 42 adult male Sprague–Dawley rats. Sepsis was induced through CLP. A bolus 30 mg/kg methylprednisolone administration followed by intravenous injection of 5.4 mg/kg/hour for 8 hours was given immediately after CLP in septic and sham-operated rats. Control groups for both septic and sham-operated rats were injected with equal volumes of saline.

Results

Neurological function was assessed at 8, 12, and 24 hours after sepsis induction. Those rats surviving for 24 hours post procedure were anesthetized and decapitated for investigation of BBB integrity using a spectrophotometric assay of Evans blue dye extravasations. A significant decrease in neurological function and a significant increase in BBB permeability were observed with the induction of sepsis compared with the sham-operated controls. Administration of methylprednisolone caused a significantly improved reflex responsiveness and decreased brain tissue Evans blue dye content in septic rats.

Conclusion

The present investigation shows that methylpred-nisolone administered immediately after sepsis induction via CLP is associated with better neurologic scores, which might be related to its positive effects on sepsis-induced BBB permeability changes.

Copyright information

© Esen et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

Authors and Affiliations

  • F Esen
    • 1
  • T Erdem
    • 1
  • A Ogan
    • 2
  • E Senturk
    • 1
  • P Ergin Özcan
    • 1
  • M Kaya
    • 1
  • N Cakar
    • 1
  • L Telci
    • 1
  1. 1.University of IstanbulTurkey
  2. 2.International HospitalIstanbulTurkey

Personalised recommendations