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Critical Care

, 11:P4 | Cite as

Effects of hypertonic saline and lactated Ringer's solutions on bacterial translocation in a rat model of intestinal obstruction and ischemia

  • FL Zanoni
  • KV Greco
  • ACR Moreno
  • LF Poli de Figueiredo
  • MR Silva
  • P Sannomiya
Poster presentation
  • 1.6k Downloads

Keywords

Ischemia Ileal Intestinal Obstruction Sodium Pentobarbital Hypertonic Saline 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Introduction

Clinical evidence suggests that bacterial translocation (BT) may not be the primary cause in the development of sepsis and multiple organ dysfunction. However, BT has an important role in the activation of the immune system. Therapies have been extensively investigated to improve tissue perfusion and reduce intestinal ischemia. The aim of this study is to evaluate the effects of hypertonic saline (HSS) 7.5% and lactated Ringer's (LR) solutions on intestinal BT in rats that underwent intestinal obstruction and ischaemia (IO).

Methods

Wistar rats (300 ± 50 g) underwent anesthesia with sodium pentobarbital (50 mg/kg, i.p.) and were submitted to IO: (i) cecum exposure, (ii) ileum ligation at 1.5 cm proximal to the ileocecal valve, and (iii) ligation of the mesenteric vessels that supply a 7–10 cm length of the ileal loop. Two hours after surgical procedures, 4 ml/kg of 7.5% HSS or LR were administered intravenously, during 5 minutes. Animals were killed 24 hours after IO, and microbiological assays were performed in mesenteric lymph nodes, liver, spleen, and blood.

Results

See Table 1.
Table 1

Microbiological assays

 

Mesenteric lymph nodes

Liver

Spleen

Blood

Group

+/n

CFU/g

+/n

CFU/g

+/n

CFU/g

+/n

Sham

1/7

57

0/7

NG

0/7

NG

0/7

IO

6/7

2,939 ± 1,751

6/7

953 ± 525

6/7

4,616 ± 1,973

4/7

LR

7/7

1,862 ± 1,178

5/7

3,080 ± 1,832

6/7

4,376 ± 2,836

6/7

HSS

6/7

2,371 ± 1,451

3/7

104 ± 67

4/7

174 ± 75

1/7

Sham group, false operated; +/n, number of animals with positive cultures for E. coli/total number of animals; CFU/g, colony formation units/g tissue (mean value ± SEM, n = 7 animals in each group); NG, no growth.

Conclusion

HSS reduced the number of CFU/g in the liver, spleen, and blood after IO, resulting in improvement of the 'gut barrier function'.

Copyright information

© BioMed Central Ltd 2007

Authors and Affiliations

  • FL Zanoni
    • 1
  • KV Greco
    • 1
  • ACR Moreno
    • 2
  • LF Poli de Figueiredo
    • 1
  • MR Silva
    • 1
  • P Sannomiya
    • 1
  1. 1.Research Division, Heart Institute (InCor), LIM 11University of São Paulo Medical SchoolSão PauloBrazil
  2. 2.Faculty of Pharmaceutical Sciences, Department of Clinical AnalysisUniversity of São PauloBrazil

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