Glucosamine enhances heat shock protein 70 expression in vitro and in vivo following injury
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KeywordsHeat Stress Heat Shock Protein Glucosamine Hemorrhagic Shock HSP70 Expression
Enhanced activity of the O-glycosylation pathway (O-glcNAc) has been shown to enhance increase heat shock protein (HSP70) expression. Glucosamine (GA) is a vital intermediate in this pathway.
Mouse fibroblast (MEF) cells underwent heat stress (HS) at 43°C for 45 minutes. GA doses from 1.25 to 20 mM were given immediately prior to HS. Cell survival was assessed via MTS assay. GA's effect on HSP70 expression in vivo was assessed using a mouse model of cecal ligation and puncture (CLP). Mice were given 0.26 g/kg GA i.v. 1 hour post CLP.
To our knowledge, this is the first report that shows GA treatment can increase HSP70 expression both in vivo and in vitro. Previous data have demonstrated beneficial effects of GA treatment following ischemia/reperfusion injury and hemorrhagic shock early after injury. GA's effect on HSP70 expression in multiple tissues may help to explain these effects. Further, GA's effect on HSP70 expression may be an important factor involved in GA's benefits in arthritis and joint disease.