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Critical Care

, 9:P41 | Cite as

In vivo leukocyte–endothelium interactions in rat mesenteric microvessels after ischemia/reperfusion and sepsis

  • NK Nakagawa
  • RA Nogueira
  • S Sinosaki
  • SR Shiwa
  • LF Poli de Figueiredo
  • P Sannomiya
  • M Rocha e Silva
Poster presentation
  • 578 Downloads

Keywords

Pentobarbital Systemic Inflammatory Response Syndrome Sodium Pentobarbital Hemorrhagic Shock Remarkable Event 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Objective

A leukocyte–endothelium interaction is known to be a remarkable event at the beginning of systemic inflammatory response syndrome. The aim of this study was to evaluate leukocyte–endothelium interactions in superfused mesenteric postcapillary venules after hemorrhagic shock/reperfusion and cecal ligation and puncture in rats.

Methods

Thirty-six Wistar rats (200–250 g) were submitted to the following interventions: 0 hours, anesthesia with sodium pentobarbital (50 mg/kg i.p.), hemorrhagic shock (MAP ~40 mmHg lasting 1 hour) and reperfusion with lactated Ringer's solution (3 × shed blood) + 25% of the shed blood; 24 hours, anesthesia and cecal ligation and puncture; 48 hours, anesthesia, cecal resection and peritoneal lavage; and 72 hours, anesthesia and intravital microscopy of the mesentery (venule diameter, 15–25 μm).

Results

Data of leukocyte–endothelium interactions in rat mesenteric microcirculation are presented as the mean ± standard deviation (Table 1).
Table 1

Leukocyte–endothelium interactions in rat mesenteric postcapillary venules

Group

n

Rolling cells/10 min

Adherent cells/100 μm venule length

Migrated cells/5000 μm2

SHAM

4

100 ± 13

3 ± 1

2 ± 1

   + CLP

6

215 ± 25*

15 ± 1*

15 ± 1*

   + CLP + REL

3

106 ± 13

5 ± 1

5 ± 1

HS + 25%

4

215 ± 14*

14 ± 2*

16 ± 1*

   + CLP

5

219 ± 9*

20 ± 2*

16 ± 1*

   + CLP + REL

4

105 ± 12

8 ± 1

8 ± 1

HS + LR

3

175 ± 10*

12 ± 0*

13 ± 1*

   + CLP

3

207 ± 16*

16 ± 1*

16 ± 1*

   + CLP + REL

4

102 ± 12

4 ± 1

15 ± 1*

HS, hemorrhagic shock; 25%, reinfusion of 25% of the shed blood volume; LR, lactated Ringer's solution; CLP, cecal ligation and puncture; REL, cecal resection and peritoneal lavage. * P < 0.01 compared with SHAM.

Conclusions

The double-hit model (ischemia/reperfusion and sepsis) induced a severe inflammatory injury similar to sepsis alone. The inflammatory process was overcome by cecal resection and peritoneal lavage. Up to 72 hours of reperfusion with lactated Ringer's solution and 25% of the shed blood volume, inflammation is still evidenced by the increased number of migrated cells in the perivascular tissue.

Notes

Acknowledgements

Supported by PRONEX, FAPESP and UNICID.

Copyright information

© BioMed Central Ltd 2005

Authors and Affiliations

  • NK Nakagawa
    • 1
  • RA Nogueira
    • 1
  • S Sinosaki
    • 1
  • SR Shiwa
    • 1
  • LF Poli de Figueiredo
    • 1
  • P Sannomiya
    • 1
  • M Rocha e Silva
    • 1
  1. 1.Research Division, Heart Institute (InCor)University of São Paulo Medical SchoolSão PauloBrazil

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