Critical Care

, 9:P41 | Cite as

In vivo leukocyte–endothelium interactions in rat mesenteric microvessels after ischemia/reperfusion and sepsis

  • NK Nakagawa
  • RA Nogueira
  • S Sinosaki
  • SR Shiwa
  • LF Poli de Figueiredo
  • P Sannomiya
  • M Rocha e Silva
Poster presentation
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Keywords

Pentobarbital Systemic Inflammatory Response Syndrome Sodium Pentobarbital Hemorrhagic Shock Remarkable Event 

Objective

A leukocyte–endothelium interaction is known to be a remarkable event at the beginning of systemic inflammatory response syndrome. The aim of this study was to evaluate leukocyte–endothelium interactions in superfused mesenteric postcapillary venules after hemorrhagic shock/reperfusion and cecal ligation and puncture in rats.

Methods

Thirty-six Wistar rats (200–250 g) were submitted to the following interventions: 0 hours, anesthesia with sodium pentobarbital (50 mg/kg i.p.), hemorrhagic shock (MAP ~40 mmHg lasting 1 hour) and reperfusion with lactated Ringer's solution (3 × shed blood) + 25% of the shed blood; 24 hours, anesthesia and cecal ligation and puncture; 48 hours, anesthesia, cecal resection and peritoneal lavage; and 72 hours, anesthesia and intravital microscopy of the mesentery (venule diameter, 15–25 μm).

Results

Data of leukocyte–endothelium interactions in rat mesenteric microcirculation are presented as the mean ± standard deviation (Table 1).
Table 1

Leukocyte–endothelium interactions in rat mesenteric postcapillary venules

Group

n

Rolling cells/10 min

Adherent cells/100 μm venule length

Migrated cells/5000 μm2

SHAM

4

100 ± 13

3 ± 1

2 ± 1

   + CLP

6

215 ± 25*

15 ± 1*

15 ± 1*

   + CLP + REL

3

106 ± 13

5 ± 1

5 ± 1

HS + 25%

4

215 ± 14*

14 ± 2*

16 ± 1*

   + CLP

5

219 ± 9*

20 ± 2*

16 ± 1*

   + CLP + REL

4

105 ± 12

8 ± 1

8 ± 1

HS + LR

3

175 ± 10*

12 ± 0*

13 ± 1*

   + CLP

3

207 ± 16*

16 ± 1*

16 ± 1*

   + CLP + REL

4

102 ± 12

4 ± 1

15 ± 1*

HS, hemorrhagic shock; 25%, reinfusion of 25% of the shed blood volume; LR, lactated Ringer's solution; CLP, cecal ligation and puncture; REL, cecal resection and peritoneal lavage. * P < 0.01 compared with SHAM.

Conclusions

The double-hit model (ischemia/reperfusion and sepsis) induced a severe inflammatory injury similar to sepsis alone. The inflammatory process was overcome by cecal resection and peritoneal lavage. Up to 72 hours of reperfusion with lactated Ringer's solution and 25% of the shed blood volume, inflammation is still evidenced by the increased number of migrated cells in the perivascular tissue.

Notes

Acknowledgements

Supported by PRONEX, FAPESP and UNICID.

Copyright information

© BioMed Central Ltd 2005

Authors and Affiliations

  • NK Nakagawa
    • 1
  • RA Nogueira
    • 1
  • S Sinosaki
    • 1
  • SR Shiwa
    • 1
  • LF Poli de Figueiredo
    • 1
  • P Sannomiya
    • 1
  • M Rocha e Silva
    • 1
  1. 1.Research Division, Heart Institute (InCor)University of São Paulo Medical SchoolSão PauloBrazil

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