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Critical Care

, 9:P200 | Cite as

Rosiglitazone, a peroxisome proliferator-activated receptor-gamma agonist, attenuates intercellular adhesion molecule-1 and cytokine-induced neutrophil chemoattractant-1 expression in lungs of rats with acute lung injury

  • D Liu
  • B Zeng
  • S Zhang
  • Z Geng
Poster presentation
  • 838 Downloads

Keywords

Acute Lung Injury Rosiglitazone GW9662 Antagonist GW9662 DMSO Control Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Aim

To investigate the effects of rosiglitazone (ROSI), a peroxisome proliferator-activated receptor-gamma (PPAR-γ) agonist, on the lung expression of intercellular adhesion molecule-1 (ICAM-1) and cytokine-induced neutrophil chemoattractant-1 (CINC-1) in rats with acute lung injury.

Methods

Thirty-six anesthetized male Wistar rats were randomly divided into six groups: DMSO control group, ROSI group, GW9662 group, lipopolysaccharide (LPS) group, ROSI + LPS group and GW9662 + ROSI group. Rats received either LPS (6 mg/kg intravenously) or vehicle (saline, 2 ml/kg intravenously). ROSI (0.3 mg/kg intravenously) or vehicle (10% DMSO) was administered 30 min before treatment with LPS. The selective PPAR-γ antagonist, GW9662 (0.3 mg/kg intravenously), was given 20 min before ROSI. Four hours after LPS injection, the wet/dry lung weight (W/D) ratio, myeloperoxidase (MPO) activity, malondialdehyde (MDA) and CINC-1 concentrations were assayed in the lung tissues. Immunohistochemical analysis of ICAM-1 expression was also studied.

Results

Pretreatment with ROSI attenuated LPS-induced increases of the W/D ratio, MPO activity, MDA and CINC-1 concentrations as well as the expression of ICAM-1 in the lung tissues (P < 0.01). The specific PPAR-γ antagonist GW9662 significantly antagonized the effects of ROSI.

Conclusion

Pretreatment with ROSI significantly reduces endotoxin-induced acute lung injury in rats; the mechanism may be through activation of PPAR-γ accompanied by inhibition of ICAM-1 and CINC-1 expression.

Copyright information

© BioMed Central Ltd 2005

Authors and Affiliations

  • D Liu
    • 1
  • B Zeng
    • 1
  • S Zhang
    • 1
    • 2
  • Z Geng
    • 2
  1. 1.Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuHanChina
  2. 2.Lanzhou General Hospital, Lanzhou Command of PLALanZhouChina

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