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Critical Care

, 19:P85 | Cite as

Evaluation of the microbial tightness of closed system transfer devices by simulating airborne and touch contamination

Open Access
Poster presentation

Keywords

Catheter Sodium Chloride Operating Room Staphylococcus Aureus Bloodstream Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Introduction

The use of intravascular catheter devices is often associated with serious bloodstream infections due to microbial contaminations. To minimize risk of such infections NIOSH recommends the use of closed system transfer devices (CSTDs). To evaluate the microbial tightness of CSTDs we developed two methods which simulate the bioburden in ambient air of operating rooms and ICUs.

Methods

The methods simulate airborne and touch contamination. We tested the microbial tightness of the integrated Safeflow® valve of a Mini-Spike® which is used for drug admixture. The airborne contamination was done in an exposure chamber in which a nebulizer distributed defined B. subtilis spore aerosols [1]. A Mini-Spike® was inserted into a vial of 0.9% sodium chloride solution (NaCl). A nebulizer with a suspension of 4.8 × 105 CFU spores of B. subtilis per ml was used to generate an aerosol for 1 minute. The volume of B. subtilis suspension nebulized per minute was 0.278 ml. This corresponds to 1.34 × 103 aerosolized spores in the exposure chamber, which has a volume of 0.24 m3 (5.6 × 103 CFU per m3 air). The used concentration was 100 times higher than the microbial burden found in hospitals [2]. After nebulization the valve was disinfected and NaCl was withdrawn into a syringe at certain time intervals. The NaCl was incubated on tryptic soy agar at 37°C for 48 hours. Results were documented as CFU. For touch contamination, a Mini-Spike® was attached to a vial of NaCl. The valve of the Mini-Spike® was contaminated with 105 CFU Staphylococcus aureus. The subsequent procedure was done as described above.

Results

Out of nine tested valves, none showed transmission of B. subtilis spores after airborne contamination. Three out of nine tested valves were contaminated with S. aureus after touch contamination.

Conclusion

Our study shows that both Methods are suitable for evaluating the microbial tightness of CSTDs.

References

  1. 1.
    Dunkelberg H, Fleitmann-Glende F: Measurement of the microbial barrier effectiveness of sterilization containers in terms of the log reduction value for prevention of nosocomial infections. Am J Infect Control. 2006, 34: 285-9. 10.1016/j.ajic.2005.09.012.CrossRefPubMedGoogle Scholar
  2. 2.
    Qudiesat K: Assessment of airborne pathogens in healthcare settings. AJMR. 2009, 3: 66-76.Google Scholar

Copyright information

© Gebel; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • J Gebel
    • 1
  1. 1.University of BonnGermany

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