Multiple trauma is linked with reversal of immunoparalysis and provides survival benefit from Pseudomonas aeruginosa
KeywordsPseudomonas Aeruginosa Pneumothorax Innate Immune Response Infected Mouse Multiple Trauma
Patients with multiple injuries are prone to hospital- acquired infections. We hypothesized that exposure to multiple injuries may modulate the innate immune response and the subsequent outcome of infections.
Ninety-seven C56Bl6 male mice were subject to multitrauma after crush of the femur and chemical pneumothorax by turpentine. Mice surviving 72 hours after the injuries were challenged intravenously with one 7log10 log-phase inoculum of P. aeruginosa and survival was recorded. In separate experiments, mice were sacrificed post injury; splenocytes were isolated and stimulated with 10 ng/ ml LPS and cytokines were measured in supernatants by an enzyme immunoassay. Quantitative cultures of the right lung, kidney and liver were performed. The same procedures were done for sham-operated mice and for mice subject only to femur crush and to pneumothorax.
Mice survivors from multiple trauma become resistant to subsequent infection. This is probably linked with the induction of tolerance of the innate immunity to substances released after sterile tissue injury.
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