Taurine chloramine decreases cell viability and cytokine production in blood and spleen lymphocytes from septic rats submitted to sepsis
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KeywordsTaurine Cytokine Secretion Hypochlorous Acid Cecal Ligation Polymorphonuclear Leucocyte
Attention has been paid in recent years to studies showing immune cell death mechanisms during the course of sepsis in response to proinflammatory and anti-inflammatory mediators that are involved in its pathophysiology. Taurine (Tau) is an abundant amino acid in polymorphonuclear leucocytes that reacts with hypochlorous acid to form taurine chloramine (TauCl) under inflammatory conditions. In this context, we investigated potential interactions between lymphocytes and TauCl in rats submitted to cecal ligation and perforation (CLP), analyzing cell viability and cytokine secretion profile (TNFα, IFNγ, IL-6, IL-17A, IL-23 and IL-10).
Materials and methods
Adult male rats were divided in two groups: sham and CLP that were killed 24 or 120 hours after sepsis induction to isolate lymphocytes from the blood and spleen. Lymphocytes (>95.0% purity determined by differentiation with Giemsa staining) were cultured for 24 hours at a concentration of 1 × 106 cells/ml and activated by 2 mg/ml concanavalin A. After 24 hours, Tau and TauCl were added at concentrations of 0.1, 0.2, 0.3, 0.4 and 0.5 mM for 1 hour. After this time, cells were incubated with MTT (500 μg/ml) for 3 hours to evaluate cell viability and supernatants were used to determine cytokine concentrations.
These findings show a possible impairment in lymphocyte function promoted by TauCl, correlated with immunosuppression and cell death characteristic of the late stages of sepsis.
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