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Critical Care

, 17:P48 | Cite as

Evaluation of recognition and signalling receptors on the peripheral blood cells of septic patients and their correlation with clinical outcomes

  • SC Silva
  • GL Baggio-Zappia
  • MKC Brunialti
  • E Silva
  • LCP Azevedo
  • FR Machado
  • R Salomao
Poster presentation
  • 427 Downloads

Keywords

Public Health Blood Sample Clinical Outcome Blood Cell Healthy Volunteer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Introduction

Monocytes and neutrophils play a key role in host defence by sensing and destroying microorganisms [1]. We evaluated the expression of cellular receptors implicated in pathogen recognition, cell activation and migration on both cell types during sepsis. Blood samples were collected from 77 septic patients (SP) at admission (D0), from 45 patients after 7 days of therapy (D7) and from 40 healthy volunteers (HV).

Results

The expression of CD14 on monocytes and of CD11b and CXCR2 on neutrophils from SP was lower than that from HV. Conversely, the expression of TLR5 on monocytes and neutrophils was higher in SP when compared with HV. The expression of TLR2 on the surface of neutrophils and that of TLR5 on monocytes and neutrophils of SP was lower at D7 than at D0. In addition, the SP that survived showed reduced expression of TLR2 and TLR4 on the surface of neutrophils at D7 compared with D0 (Figure 1). The expression of CXCR2 for surviving patients was higher at follow-up when compared with baseline (Figure 1).
Figure 1

Analysis of the expression of TLR2 (A), TLR4 (B) and CXCR2 (C) surface receptors on neutrophils from the whole blood of surviving septic patients and nonsurvivors at D0 and D7. Histograms and expressed as the geometric mean fluorescence intensity (GMFI). * P < 0.05.

Conclusion

The expression of recognition and cell signalling receptors is differentially regulated between SP and HV depending on the receptor being evaluated. However, despite these changes, it is likely that the functional changes in monocytes and neutrophils that are observed during sepsis are not directly linked to the modulation of the expression of TLRs [2].

References

  1. 1.
    Ley K: Molecular mechanisms of leukocyte recruitment in the inflammatory process. Cardiovasc Res 1996, 32: 733-742.CrossRefPubMedGoogle Scholar
  2. 2.
    Salomao R, Brunialti MK, Rapozo MM, Baggio-Zappia GL, Galanos C, Freudenberg M: Bacterial sensing, cell signaling, and modulation of the immune response during sepsis. Shock 2012, 38: 227-242. 10.1097/SHK.0b013e318262c4b0CrossRefPubMedGoogle Scholar

Copyright information

© Silva et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • SC Silva
    • 1
  • GL Baggio-Zappia
    • 1
  • MKC Brunialti
    • 1
  • E Silva
    • 2
  • LCP Azevedo
    • 3
  • FR Machado
    • 1
  • R Salomao
    • 1
  1. 1.Hospital Sao Paulo, Escola Paulista de Medicina, Universidade Federal deSao PauloBrazil
  2. 2.Hospital Israelita Albert EinsteinSao PauloBrazil
  3. 3.Hospital Sirio LibanesSao PauloBrazil

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