Advertisement

Critical Care

, 5:P076 | Cite as

Moderate hypothermia during cardiac surgery provides hepatic protection by modifying the balance between gene expression of TNFα and IL10

  • M Qing
  • JF Vazquez-Jimenez
  • B Klosterhalfen
  • K Schumacher
  • BJ Messmer
  • G von Bernuth
  • M-C Seghaye
Meeting abstract

Keywords

Gene Expression Apoptotic Cell Regulatory Protein Cardiopulmonary Bypass Hepatic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Aims

To investigate the effect of moderate hypothermia during CPB on intrahepatic gene expression of TNFα, IL10, apoptosis regulatory proteins (Fas, Bak and Bcl-xL) and hepatic cell death during and after cardiac surgery.

Methods

Pigs were assigned to a temperature (T°) group during standardized cardiopulmonary bypass (CPB): normothermia (T°, 37°C; n = 8) and moderate hypothermia (T°, 28°C; n = 8). Liver probes were taken before and 6 hours after CPB for standard-and immunohistological examinations. Apoptotic cells were detected by TUNEL-staining. Intrahepatic gene expression of TNFα, IL10 and of apoptosis regulating proteins were examined by competitive RT-PCR.

Results

Gene expression of cytokine and apoptosis regulating proteins was not detected before but 6 hours after CPB. Pigs operated on under 28°C showed lower TNFα-mRNA and higher IL10-mRNA than those operated on under 37°C (P < 0.05). While expression of apoptosis regulatory proteins and percentage of apoptotic hepatocytes were similar in both groups, percentage of necrotic hepatocytes was lower in 28°C than in 37°C group (P < 0.05). TNFα-mRNA after CPB was correlated with the percentage of necrotic hepatocytes (P < 0.05).

Conclusions

Moderate hypothermia during CPB provides hepatic protection by increasing IL10- and decreasing intrahepatic TNFα-gene expression without affecting gene expression of apptosis regulatory proteins.

Copyright information

© The Author(s) 2001

Authors and Affiliations

  • M Qing
    • 1
  • JF Vazquez-Jimenez
    • 2
  • B Klosterhalfen
    • 3
  • K Schumacher
    • 1
  • BJ Messmer
    • 2
  • G von Bernuth
    • 1
  • M-C Seghaye
    • 1
  1. 1.Department of Pediatric CardiologyUniversity of Technology,University HospitalAachenGermany
  2. 2.Department of Cardiovascular Surgery,Department of and PathologyAachen UniversityHospitalAachenGermany
  3. 3.Department of and PathologyAachen UniversityHospitalAachenGermany

Personalised recommendations