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Minimal residual disease (MRD) in the bone marrow in ER-α-positive primary breast cancer patients

  • RC Coombes
  • MT De Bella
  • G Tripuraneni
  • A Zaidi
  • S Anwer
  • DA Stephens
  • B Ward
  • HD Sinnett
  • MJ Slade
Oral presentation
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Keywords

Breast Cancer Bone Marrow Primary Breast Cancer Minimal Residual Disease Estrogen Action 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Introduction

We conducted a study to determine whether a group of estrogen-induced genes could be used to detect and monitor for micrometastases in the bone marrow of patients with breast cancer.

Methods

We data-mined for potential markers of estrogen action, verified their relationship to ER in cell lines and purified cells from patient biopsies, and checked their estrogen-inducibility after developing a real-time quantitative PCR assay for each. We then examined 99 bone marrow samples obtained over 2 years during the follow up of good (n = 7) or poor (n = 19) prognosis patients to determine the expression frequency.

Results

We discovered that the expression of eight out of 23 genes, identified by data-mining, were estrogen-regulated. We developed real-time quantitative PCR (QPCR) assays for measurement of the genes for which ESTs were available (ER-α, PR and GATA-3, EEIG-1, EP-3, PS2). We examined their expression in purified breast cancer cells from primary cancers and also from metastases from endocrine-resistant cancers and confirmed that these genes were still expressed. Of these, three were expressed in peripheral blood, excluding them as candidate markers. We then examined 79 samples of bone marrow from 19 poor prognosis patients and 20 from seven good prognosis patients. We found that GATA-3 and ER expressions were significantly higher in the bone marrow of poor-prognosis patients.

Conclusion

GATA-3 and ER appear to be potentially useful markers, in addition to CK19, for monitoring the effects of treatment in the bone marrow of patients with ER-positive breast cancer.

Notes

Acknowledgements

This work was funded by the Breast Cancer Research Trust (MTDB) (MS) and Cancer Research (UK) (RCC).

Copyright information

© BioMed Central 2005

Authors and Affiliations

  • RC Coombes
    • 1
    • 4
  • MT De Bella
    • 1
  • G Tripuraneni
    • 1
  • A Zaidi
    • 1
  • S Anwer
    • 1
  • DA Stephens
    • 2
  • B Ward
    • 1
  • HD Sinnett
    • 3
  • MJ Slade
    • 1
    • 4
  1. 1.Cancer Research UK LaboratoriesDepartment of Medicine, Imperial CollegeLondonUK
  2. 2.Department of Mathematics, Imperial CollegeLondonUK
  3. 3.Breast Clinic, Charing Cross Hospital, Faculty of MedicineImperial CollegeLondonUK
  4. 4.Joint senior authorsLondonUK

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