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Evidence for an antigen-driven immune response in the chronically inflamed synovium

  • Claudia Bearek
  • H-J Kim
Meeting abstract
  • 1.6k Downloads

Keywords

Rheumatoid Arthritis Arthritis Proinflammatory Cytokine Plasma Cell Lymphoid Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Full text

Recently it has been shown that proinflammatory cytokines, like TNF-alpha and lymphotoxin play a crucial role in the organogenesis of the lymphoid tissue. Thus, during chronic inflammation, in the affected tissue, these cytokines may promote the development of a micro-environment which supports a local immune response. In patients with rheumatoid arthritis large lymphocytic infiltrates are often seen in the synovial tissue. These cell clusters have a characteristic arrangement of T, B and plasma cells. Proliferating B cells are found only centrally in a network of follicular dendritic cells. Using micro-manipulation CD20+ B cells and plasma cells were isolated separately from different parts of single infiltrates. DNA was extracted and the VH/VL gene repertoires determined. The data show that within the network of follicular dendritic cells there is an oligoclonal expansion of B cells. During proliferation V-gene variants are generated by the hypermutation mechanism. The pattern of somatic mutations suggests that both naive and memory B cells become activated in the synovial tissue. Within single infiltrates we did not find identical rearrangements between CD20+ B and plasma cells. Nevertheless, the finding of clonally related plasma cells suggests that these cells underwent terminal differentiation in the synovial tissue. The analysis of individual B cells recovered from synovial tissue opens a new way to determine the specificity of those cells which take part in the local immune reaction.

References

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Copyright information

© Current Science Ltd 2000

Authors and Affiliations

  • Claudia Bearek
    • 1
  • H-J Kim
    • 1
  1. 1.Deutsches Rheuma ForschungsZentrumBerlinGermany

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