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Tolerogenic dendritic cells differentially modulate naïve and memory CD4+ T cells

  • Amy E Anderson
  • Bethan Sayers
  • Julie Diboll
  • John D Isaacs
  • Catharien MU Hilkens
Poster presentation

Keywords

Public Health Dendritic Cell Dexamethasone Cytokine Production Modulative Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Background

Dendritic cells (DC) can be alternatively activated to induce tolerogenic DC, making them a promising therapy for autoimmunity. Because naïve and memory T cells have different requirements for tolerisation, we tested the modulatory activity of tolerogenic DC on both subsets of T cells.

Methods

Human monocyte-derived DC were matured with lipopolysaccharide (LPS-DC) or treated with dexamethasone, vitamin D3 and lipopolysaccharide (LPS-DexD3 DC) to obtain tolerogenic DC. DC were cocultured with allogeneic naïve or memory CD4+ T cells. Primed T cells were rested and restimulated with LPS-DC or CD3/CD28 beads.

Results and conclusions

LPS-DexD3 DC have reduced stimulatory capacity for both naïve and memory T cells. However, restimulation of T cells revealed a distinct difference between naïve and memory T cells that had been primed by LPS-DexD3 DC. Naïve T cells did not become anergic but were skewed to a regulatory phenotype (low IFNγ and high IL-10 production), whereas memory T cells were rendered hyporesponsive, with low proliferation and cytokine production. Thus, naïve and memory T cells are differently regulated by LPS-DexD3 DC. These data have implications for the use of tolerogenic DC vaccines as immunomodulators.

Copyright information

© BioMed Central Ltd 2007

Authors and Affiliations

  • Amy E Anderson
    • 1
  • Bethan Sayers
    • 1
  • Julie Diboll
    • 1
  • John D Isaacs
    • 1
  • Catharien MU Hilkens
    • 1
  1. 1.Clinical Immunotherapy Group, Department of RheumatologyNewcastle UniversityUK

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