Advertisement

Arthritis Research & Therapy

, 3:P047 | Cite as

The release of an ERK-activating factor from cartilage explants in response to trauma

  • T Vincent
  • M Bolton
  • J Saklatvala
Meeting abstract

Keywords

Public Health Arthritis Degenerative Arthritis Articular Cartilage Mitogen Activate Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Mechanical injury to cartilage predisposes to premature degenerative arthritis but little is understood of the chondrocytic response to injury at the cellular and molecutar level. We have shown that the extracellular regulated kinase (ERK), the original mitogen activated protein (MAP) kinase, is strongly activated in porcine articular cartilage upon scarification in vivo, or following cutting of rested cartilage explants in vitro. Activation occurs within minutes and is sustained for 24-48 h. It appears to be mediated by a soluble factor which is released into the culture medium by damaged cartilage. The factor is thermolabile and retained by dialysis membrane (10 kDa cut-off). We have purified the factor through a series of chromatography steps (anion and cation exvhange, gel filtration and agarose-heparin columns), and are awaiting identification. It represents a potential homeostatic mechanism in response to injury, and could play a role in normal metabolism and degenerative arthritis.

Copyright information

© BioMed Central Ltd 2001

Authors and Affiliations

  • T Vincent
    • 1
  • M Bolton
    • 1
  • J Saklatvala
    • 1
  1. 1.Kennedy Institute of RheumatologyLondonUK

Personalised recommendations