The Use of Fa-Technique for Detecting Francisella Tularensis in Formalin Fixed Material

A Method Useful in Routine Post Mortem Work

Användningen av FA-teknik för att påvisa Francisella tularensis i formalinfixerat material

En anvåndbar metod i rutindiagnostik

Abstract

Tularemia is a highly infectious zoonosis, in Sweden usually occurring in the varying hare. The risk of human infections when diagnosing this disease in the laboratory is high. Therefore, a method for diagnosing tularemia in formalin fixed material with the FA-technique has been tested. 46 necropsied varying hares were examined using this method. 28 of the examined animals were diagnosed with tularemia based on conventional post mortem, histological and bacteriological examinations. 96 per cent of them were positive to the FA-technique test. 18 other animals infected with different bacteria and Toxoplasma were also tested. All the controls were negative.

Sammendrag

Tularemi är en mycket smittsam zoomens som i Sverige vanligen uppträder hos skogshare. Risken för att laboratoriepersonal skall smittes vid obduktion och diagnostik är stor. Det är därfär önskvärt att kunna diagnostisera sjukdomen på icke smittsamt material. En metod att undersöka formallinfixerat material med hjälp av fluorescentantikroppsteknik har genomförts.

46 harar obducerade vid SVA har ingått i denna studie. 28 av dessa har diagnostiserats som tularemi gruradat på patolog-anatomisk bild, histologi och bakteriologi. 27 st (96 %) av dessa var positiva med FA-testen. Resterande 18 obducerade harar med olika bakteriella infektioner eller infekterade med toxoplasmos testades med FA-testen. Alla fall testade med kontrollserum var negativa.

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Correspondence to Torsten Mörner.

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Mörner, T. The Use of Fa-Technique for Detecting Francisella Tularensis in Formalin Fixed Material. Acta Vet Scand 22, 296–306 (1981). https://doi.org/10.1186/BF03548655

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Keywords

  • FA-technique
  • Francisella tularensis
  • post mortem work
  • varying hare