Corynebacterium Pseudotuberculosis Infection in Goats VII.

Corynebacterium pseudotuberculosis infeksjon hos geit VII. Kliniske, patologiske, serologiske og hematologiske forandringer etter subcutan inokulering av bakterier.

Clinical, Pathological, Serological and Hematological Changes after Subcutaneous Inoculation of the Organism

Abstract

Six castrated male kids from 2 herds free from caseous lymphadenitis, were inoculated subcutaneously with about 1 million colony forming units of a Corynebacterium pseudotuberculosis strain isolated from goat. The animals exhibited a febrile response and marked inflammatory reaction at the inoculation site during the first days after infection. Post mortem examination carried out 2 months after inoculation revealed abscesses in the regional lymph node (Inn. subiliaci) of all animals, in 3 of which lesions also appeared in other lymph nodes. The antibody titre (mean value) in the bacterial agglutination test peaked during the first 2 weeks following inoculation, while the titre in the hemolysis inhibition test began to rise from 2–3 weeks after the infection and continued to increase during the rest of the investigation period (9 weeks after inoculation). Hemoglobin and hematocrit levels decreased significantly after inoculation, and remained below the preinfection levels for 2 weeks. A significant leucocytosis was also seen during the same period.

The present investigation indicates that subcutaneous inoculation with C. pseudotuberculosis might be a suitable challenge system to study the efficacy of vaccines against caseous lymphadenitis in goats.

Sammendrag

Seks kastrerte bukkekje fra 2 besetninger fri for kaseøs lymfadenitt ble inokulert subcutant med ca. 1 million kolonidannende enheter av en Corynebacterium pseudotuberculosis stamme isolert fra geit. Dyrene fikk feber og en uttalt betennelse på inokuleringsstedet de første dagene etter inokulering. Obduksjon ble foretatt 2 måneder etter belastning, og alle dyrene hadde da abscesser i den regionale lymfeknuten (lnn. subiliaci). Hos 3 kje ble det også påvist lesjoner i andre lymfeknuter. Antistofftiteret (gjennomsnittsverdien) målt ved bakterieagglutinasjonstest økte til høyeste verdi i løpet av de 2 første ukene etter inokulering, mens det ved antihemolysintest økte fra 2 uker etter infeksjon og resten av undersøkelsesperioden (9 uker etter inokulering). Hematokritt- og hemoglobinverdiene avtok etter inokulering og var lavere enn før inokulering i de 2 følgende ukene. I samme periode ble det påvist leucocytose hos dyrene. Det konkluderes med at det er hensiktsmessig å benytte subcutan belastning med C. pseudotuberculosis til å studere effektivsten til vaksiner mot kaseøs lymfadenitt hos geit.

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Acknowledgments

We thank the Department of Medicine 1, Norwegian College of Veterinary Medicine, for assistance in performing the hematological examina-tions. The investigation was supported by grants from the Agricultural Research Council of Norway.

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Correspondence to Gudmund Holstad.

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Holstad, G., Teige, J. Corynebacterium Pseudotuberculosis Infection in Goats VII.. Acta Vet Scand 29, 287–294 (1988). https://doi.org/10.1186/BF03548620

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Keywords

  • experimental infection
  • inflammation
  • abscesses
  • antibodies
  • hemoglobin
  • hematocrit
  • leucocytes.