Tissue Localization of DDT and Two PCB Isomers (Octa- and Tetra-Chlorobiphenyl) in Laying Quails

Vavnadslokalisation av DDT och två PCB-isomerer (okta- och tetra-klorbifenyl) i värpande vaktelhöna

Abstract

Whole-body autoradiography of DDT-14G, 2,2′,4,5′-tetrachlorobiphenyl-14G and 2,2′,3,3′,4,4′,5,6′-octachlorobiphenyl-14C in laying quails indicated a strong deposition of radioactivity in the yolk of the growing follicles and the egg, confirming this to be a significant excretion route of lipophilic environmental pollutants in laying birds. A specific uptake of DDT and octachlorobiphenyl, but not of tetrachloro-biphenyl, was observed in the cortical cords of the adrenals. The octachlorobiphenyl was concentrated also in the ovarian stroma. Both DDT and the tetrachlorobiphenyl were excreted in the bile juice to the small intestine. No such excretion of octachlorobiphenyl was observed, indicating its rate of metabolization to be low. All compounds were accumulated and stored in the body fat. A previously described specific accumulation of 2,2′,4,5′-tetrachlorobiphenyl in the respiratory tract of mice was not observed in the quail.

Sammanfattning

Distributionen av DDT-14C, 2,2′4,5′-tetraklorbifenyl-14C och 2,2′, 3,3′,4,4′,5,6′-oktaklorbifenyl-14C studerades med helkroppsautoradio-grafi. Samtliga substanser koncentrerades i äggets och de växande folliklarnas gula, vilket visar att detta är en betydande utsöndringsväg för lipofila miljögifter hos värpande fågel. Vidare sågs en uttalad ackumulation av DDT och oktaklorbifenyl i binjurens kortikalsträngar. Oktaklorbifenyl koncentrerades dessutom i ovariets stroma. Både DDT och tetraklorbifenyl utsöndrades med galla till tarmen. Oktaklorbifenyl koncentrerades däremot ej i galla och tarm, vilket antyder att denna PGB metaboliseras långsamt. Samtliga substanser ackumu-lerades och retinerades i kroppsfett. Tetraklorbifenyl, som tidigare visats ha specifik affinitet till bronkialslemhinnan hos mus, anrikades inte i respirationsvägarna hos vaktel.

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Acknowledgments

We are indebted to Professor Carl Axel Wachtmeister and his co-workers Drs. Äke Bergman and Göran Sundström for the synthesis of the labelled polychlorobiphenyls. Miss Raili Pensas and Mr. Hans Sundberg are acknowledged for skilful technical assistance.

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Correspondence to Ingvar Brandt.

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Brandt, I., Högman, PG., Larsson, Y. et al. Tissue Localization of DDT and Two PCB Isomers (Octa- and Tetra-Chlorobiphenyl) in Laying Quails. Acta Vet Scand 19, 368–376 (1978). https://doi.org/10.1186/BF03547606

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Keywords

  • DDT
  • polychlorinated biphenyls (PCB)
  • autoradiography
  • quail
  • adrenal
  • ovary
  • yolk