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Sensitisation patterns to tomato seed

  • Miguel González
  • Laura Martín-Pedraza
  • Maria Luisa Somoza
  • Natalia Blanca-López
  • Maria Luisa Macías
  • Diana Perez
  • Mayte Villalba
  • Cristobalina Mayorga
  • Gabriela Canto
  • Maria Jose Torres
  • Ana Aranda
  • Ana Molina
  • Miguel Blanca
Open Access
Poster presentation
  • 267 Downloads

Keywords

Food Allergy Urticaria Skin Prick Test Angioedema Seed Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Rationale

Food allergy is an increasing health problem with many proteins involved that belong mainly to a limited number of families. They show a high level of cross-reactivity. In the Mediterranean area, the most prevalent food allergens are those of vegetal origin. Although tomato (Solanum lycopersium L.) is one of the implicated foods, studies on the identification and relevance of their allergens have not been carried out in detail. This could be particularly relevant for tomato seeds as happen in other fruits like kiwi.

The aimed was to analyse the sensitisation pattern to tomato seeds in patients from two hospitals integrated in the RIRAAF.

Methods

A large group of tomato-sensitized patients (N=96) was recruited. We included patients who suffered at least two episodes with tomato and/or having a positive skin prick test (SPT). Raw tomato seed extract was prepared and the protein profile characterized by SDS-PAGE. Patient sera were used for determining recognition profiles by western blotting.

Results

Data from western blotting showed different patterns of IgE recognition. From all the bands those approx. of 10 kDa was the most frequently recognised in 46% of the patients. This band specifically appeared in 100% of serum from patients with anaphylaxis, 83% with urticaria, 0% with angioedema and 9% with OAS.

Conclusions

These preliminary results show that a new seed protein from tomato could be a relevant allergen. Whether this is predictive of systemic reactions is being evaluated.

Copyright information

© González et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Miguel González
    • 1
  • Laura Martín-Pedraza
    • 2
  • Maria Luisa Somoza
    • 3
  • Natalia Blanca-López
    • 3
  • Maria Luisa Macías
    • 1
  • Diana Perez
    • 3
  • Mayte Villalba
    • 2
  • Cristobalina Mayorga
    • 1
  • Gabriela Canto
    • 3
  • Maria Jose Torres
    • 4
  • Ana Aranda
    • 1
  • Ana Molina
    • 1
  • Miguel Blanca
    • 4
  1. 1.Research Laboratory, IBIMARegional University Hospital of Malaga, UMAMalagaSpain
  2. 2.Biochemistry and Molecular Biology DepartmentUniversity Complutense MadridSpain
  3. 3.Allergy ServiceInfanta Leonor HospitalMadridSpain
  4. 4.Allergy UnitIBIMA, Regional University Hospital of Malaga, UMAMalagaSpain

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