Map-based cloning of dep. (a) Rough and fine map basis of complementation tests carried out to identify the gene responsible for the glabrous phenotype in rice. Red-, double-headed arrow indicates the 62-kb candidate region identified for dep. (b) BAC sub-clones used in the initial complementation test that restored the pubescence of the substitution line used for transformation. (c) Sub-clones generated from the LL3 fragment that complemented the glabrous phenotype in the substitution line used for transformation. (d) Annotated genes covered within the LL3scG2 sub-clone. (e) Sub-clones derived from LL3scG2 fragment that were used to transform a glabrous substitution line of rice. Lines that are marked with a cross failed to complement the glabrous phenotype whereas those marked with a check restored the pubescence in T0 plants. Figure not drawn to scale.