Linkage analysis and fine-scale mapping of dep. (a) Mapping of the dep gene on the short arm of chromosome 5. The blue bar represents the putative location of the gene. (b) Fine mapping of dep using F2 lines generated from crosses between Koshihikari and GLSL15, and F3 lines derived from F2 lines with informative recombination points. Red, double-headed arrows show the candidate region identified for each of the populations genotyped. (c) Annotated genes covered within the BAC clones spanning the 22-kb candidate region. Figure not drawn to scale.