The guidance of stem cell cardiomyogenic differentiation by bioartificial scaffolds mimicking myocardium structure and biomechanics
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KeywordsMesenchymal Stem Cell Regenerative Medicine Cardiac Differentiation Chemical Compatibility Confocal Microscopy Analysis
Despite enormous progresses in the treatment of coronary artery disease, it remains the most common cause of heart failure and the leading cause of death in the Western countries. New translational therapeutic approaches based on personalized and regenerative medicine explore cardiomyogenic differentiation of various types of stem cells by electrical stimulation, biochemical inducers, or cell co-culturing [1, 2, 3]. In this study we fabricated bioartificial constructs mimicking anisotropic structure and mechanical properties of the myocardium .
Constructs based on PHBHV and gelatin were prepared and characterized by physico-chemical, mechanical and degradation tests including FT-IR, DSC, DMA and HPLC analyses. Cell adhesion and proliferation on these constructs were evaluated at 1, 4, 8, 12, 15 days using CellTiter Blue® viability assay. Cell morphology and construct colonization was evaluated using the viable dye Calcein-AM, while cytoskeletal organization and focal adhesions was analyzed by immunofluorescence with phalloidin staining and TEM. Cardioinductivity was investigated through qPCR (gene expression) and immunofluorescence (protein expression) experiments.
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This work represents a new approach to induce both circulating and resident stem cells to differentiate toward cardiac cells in a 3-D structure, without using any additional stimuli. These constructs have the potential to serve as patches for cardiac regeneration.
Our results suggest that the two technology-driven components of the healthcare revolution, personalized medicine and regenerative medicine, offer the opportunity to address unmet medical needs.
Funding from P.O.R. F.E.S.R., 2007/2013 - under grant agreement n° 14557- is gratefully acknowledged.
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