The in vitro effect of guaraná (Paullinia cupana) extract on human peripheral blood mononuclear cells exposed to a high glucose level
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KeywordsNitric Oxide Peripheral Blood Mononuclear Cell Theophylline Antioxidant Capacity Cellular Proliferation
Nowadays, Type 2 Diabetes Mellitus (T2D) affects a significant percentage of the world population. Due to the high levels of glucose, it causes several damages to the cells and, consequently, to the organism. Plants, such as guaraná (Paullinia cupana), which present bioactive compounds, could help somehow in the treatment of diabetes and other chronic diseases. Studies have shown that guaraná extract, rich in compounds like methylxanthines, theobromine and theophylline, presents many beneficial effects to the organism, such as antioxidant capacity, modulation of nitric oxide intracellular levels, as well as anti-obesity and anti-inflammatory capacity.
The aim of this study was to evaluate the in vitro effect of guaraná (Paullinia cupana) extract on the viability and cellular proliferation of Peripheral Blood Mononuclear Cells (PBMC) exposed to a high glucose level.
Materials and methods
Guaraná powder was provided by EMBRAPA, located in the city of Maués, Amazonas. The hydroalcoholic extract was prepared following Bittencourt et al. (2013). Initially, peripheral blood from 20 young and healthy individuals (8 h of fasting) was collected. The PBMC were isolated with Ficoll-Histopaque®, cultured in RPMI 1640 medium for 72 h at 37o C and treated with the guaraná concentrations (1 mg/mL, 5 mg/mL and 10 mg/mL), glucose (15 mg/mL) or both. The control group does not received any treatment. MTT assay was performed to evaluate the cellular proliferation and PicoGreen assay for the evaluation of the cellular viability.
The results indicate that the guaraná (P. cupana) extract has a beneficial effect on the PBMC, increasing the cellular proliferation and decreasing the extracellular dsDNA levels. These data corroborate with other studies that have shown the guaraná antioxidant capacity. The guaraná extract presented statistically significant positive activity even when combined with a high level of glucose, indicating that it could be used to improve the organism functioning of healthy people as well as patients with T2D.
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