Silencing subtelomeric VSGs by Trypanosoma brucei RAP1 at the insect stage involves chromatin structure changes
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KeywordsChromatin Structure Mammalian Host Antigenic Variation Human African Trypanosomiasis Trypanosoma Brucei
Trypanosoma brucei causes human African trypanosomiasis and undergoes antigenic variation to evade its mammalian host immune attack. Throughout its life cycle, T. brucei is covered with glycoproteins on its cell surface. When infecting a mammalian host, bloodstream form (BF) T. brucei expresses bloodstream expression site (BES)-linked variant surface glycoproteins (VSGs) in a monoallelic fashion to ensure effectiveness of antigenic variation. In the midgut of its insect vector, tsetse, procyclic form (PF) T. brucei expresses procyclins. After migration to the tsetse’s salivary glands, T. brucei expresses metacyclic VSGs (mVSGs) to prepare for mammalian host infection. Regulation of VSG expression/silencing is critical for T. brucei virulence and its development. However the underlying mechanism of VSG regulation is not fully understood. Our lab identified a telomeric protein TbRAP1 as a key regulator of BES-VSGs in BF . In this study we have further elucidated role of TbRAP1 in VSG regulation at PF stage and its possible underlying mechanism through chromatin remodeling.
Materials and methods
We depleted TbRAP1 in PF cells using an inducible RNA interference (RNAi) approach. Quantitative RT-PCR was performed to analyze changes in steady state mRNA levels of BES-lined and metacyclic VSGs after depletion of TbRAP1. In order to test the effect of TbRAP1 depletion on chromatin structure, Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) analysis and Micrococcal-nuclease digestion assays were performed.
In this study, we found that depletion of Tb RAP1 by RNAi led to derepression of mVSGs at both the BF and PF stages. Similar to that observed in BF cells, silencing of BES-linked VSGs in PF cells also depends on Tb RAP1. In addition, we found that silencing of BES-linked VSGs by Tb RAP1 is stronger in PF cells than that in BF cells. Furthermore, removal of Tb RAP1 led to a loosened chromatin structure at multiple loci in PF cells, particularly at the BES loci, but not in BF cells, indicating that different silencing mechanisms are used at different stages and that Tb RAP1-mediated silencing may involve modulation of chromatin structure in PF cells.
Our observations confirm that Tb RAP1 is a key VSG silencer and that Tb RAP1 helps to determine the chromatin structure in multiple loci throughout the genome, particularly at BESs, at the insect stage.
I thank Cleveland State University Research Council’s Doctoral Dissertation Research Expense Award and Fellowship (DDREAFP) program for providing funds to attend this conference.
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