BMC Proceedings

, 8:P60 | Cite as

Cytotoxicity of Agave sisalana in hemocytes of the mosquito Aedes aegypti, which transmits dengue

  • Débora Lacerda
  • Louise Oliveira
  • Fabiola Nunes
Open Access
Poster presentation


Public Health High Resistance Viral Disease Present Symptom Severe Illness 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


The dengue is a viral disease transmitted by a mosquito from the Culicidae family from Africa, the Aedes aegypti. Most common in tropical and subtropical regions, this mosquito has diurnal activity and is difficult to control due to the high resistance of its eggs and its quick transformation into larvae. The severe illness may cause death, most commonly presenting symptoms such as high fever, pain behind the eyes and headaches.


Fifteen larvae (L4) were exposed in a 6.5 mg/mL concentration of A.sisalana juice, during the periods of 3, 6, 12 and 24 hours, completing 4 experimental groups and one control group for each, with means, 4 control groups.

Results and conclusions

The A. sisalana had a cytotoxic necrotizing effect on A. aegypti hemocytes, showing that after 12 hours of exposition the larvae in juice had a 21% hemocytes mortality compared with the control group. After 24 hours, a cell mortality of 16.5% in relation with the control group, as well the growth of hemocytes' size and granularity, being compatible with the noted necrosis. In fact, this effect was acute, because, after 24 hours, the majority of larvae were dead even when a sub-lethal concentration was used, what suggest that this is the major sisal juice action mechanism.



Biotechnology Center and Federal University of Paraiba.

Copyright information

© Lacerda et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Débora Lacerda
    • 1
  • Louise Oliveira
    • 1
  • Fabiola Nunes
    • 1
  1. 1.Biotechnology CenterFederal University of ParaibaJoão PessoaBrazil

Personalised recommendations