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BMC Proceedings

, 8:P25 | Cite as

Cell therapy in renal ischemia/reperfusion experimental model using recombinant G-CSF

  • Vinicius Correa Rodrigues
  • Isabela Bastos Binotti Araújo
  • Rosiane Ervati
  • Sônia da Penha Silva Oliveira
  • Breno Valentim Nogueira
Open Access
Poster presentation

Keywords

Urea Creatinine Renal Function Proteinuria Creatinine Clearance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Introduction

The colony stimulating factor granulocyte (G-CSF) is a glycoprotein capable of promoting the survival, proliferation and differentiation of hematopoietic cells. Studies demonstrate the cytoprotective action of G-CSF against renal injury by ischemia/reperfusion injury in murine models. But the literature is still controversial in relation to the risk of worsening renal function after useG-CSF, which motivated the study to elucidate possible interference on the renal function of the treated animals.

Objective

Evaluate the renal function of rats after treatment with G-CSF at different doses of the drug.

Methods

Male Wistar rats (n = 18), 200g approx. (CEUA050/2013), divided into 3 groups (6 animals each) : control group (C) 5% glucose solution (solvent) groups treated with G-CSF at a dose of 10 (G10) and 50 (G50) mg / kg / per 5 days. After treatment, the rats were placed in metabolic cages for urine collection and obtaining urine volume. Values were obtained from creatinine, proteinuria, urea and number of circulating leukocytes. The results were expressed as mean ± SEM. The averages ofvalues between groups were calculated using one - way ANOVA followed by post hoc Fisher for comparison between differentgroups.

Results

A significant increase in the number of circulating leukocytes in animals treated with G- CSF (C = 9687 ± 899 / mm3;= 14375 ± 1967/mm3 G10, and G50 = 19670 ± 1663/mm3, p < 0.05). There was not a significant increase in urine volume after 24 hours treatment with G-CSF. There was no significant difference between the values of creatinine clearance, proteinuria and Urea, among groups C, G10 and G50.

Conclusion

There was no impairment of renal function in animals treated at doses of 10 and 50 mg / kg / per 5 days.

Financial support: FAPES

References

  1. 1.
    Stokman G, Leemans JC, Claessen N, Weening JJ, Florquin S: Hematopoietic stem cell mobilization therapy accelerates recovery of renal function independent of stem cell contribution. J Am Soc Nephrol. 2005, 16 (6): 1684-1692. 10.1681/ASN.2004080678.CrossRefPubMedGoogle Scholar
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    Nishida M, Hamaoka K: How does GCSF act on the kidney during acute tubular injury?. Nephron Exp Nephrol. 2006, 104 (4): e123-e128. 10.1159/000094962.CrossRefPubMedGoogle Scholar
  3. 3.
    Verweij M, Sluiter W, van den Engel S, Jansen E, Ijzermans JN, de Bruin RW: Altered Mitochondrial Functioning Induced by Preoperative Fasting May Underlie Protection Against Renal Ischemia/Reperfusion Injury. Journal of Cellular Biochemistry. 2013, 114 (1): 230-237. 10.1002/jcb.24360.CrossRefPubMedGoogle Scholar

Copyright information

© Rodrigues et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Vinicius Correa Rodrigues
    • 1
  • Isabela Bastos Binotti Araújo
    • 1
  • Rosiane Ervati
    • 2
  • Sônia da Penha Silva Oliveira
    • 2
  • Breno Valentim Nogueira
    • 2
  1. 1.PG BiotecnologiaUFESVitoriaBrasil
  2. 2.Departamento de MorfologiaUFESVitoriaBrasil

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