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BMC Proceedings

, 7:P45 | Cite as

Tumor RNA-transfected dendritic cells and combined therapy with low dose 5-FU induce regression of murine colon cancer

  • Marcela R de Camargo
  • Carolina M Gorgulho
  • Cecília P Rodrigues
  • Juliana CL Frederico
  • Fabiana A Zambuzi
  • Victoria E Galvão
  • Marcimara Penitenti
  • Ramon Kaneno
Open Access
Poster presentation
  • 623 Downloads

Keywords

Dendritic Cell Paclitaxel Antitumor Response Dendritic Cell Vaccine Minimum Effective Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Background

We have recently observed that treatment of colon tumor cells with low concentration of paclitaxel increased the expression of several genes associated with antigen-presenting machinery. Since 5-fluoruracil (5-FU) is the main antineoplastic agent for colon cancer, in this study we aimed to evaluate: a) whether transfection of dendritic cells (DC) with drug-treated tumor cells RNA, enhances the effectiveness of DC-based vaccine; b) if the modulatory effects of vaccine can be observed in vivo, and c) if the combination of DC with low dose chemotherapy schedule improves the antitumor responsiveness.

Materials and methods

Murine colon cancer cells (MC-38) were treated with the minimum effective concentration (MEC) of 5-FU and their RNA was used to transfect DC. Then, C57/Bl-6 tumor-bearing mice were treated with DC vaccine. Another group of animals received low doses of chemotherapy schedule and DC vaccine.

Results

Results of 2 independent assays have shown that vaccination with RNA-transfected DC delayed the tumor growth, increased the percentage of CD86+ (35%) CD40+ (63%) and MHC class II+ (47%) and significantly increased the in vitro production of IFN-γ and decreased IL-10 by spleen cells co-culture. In addition we observed that the combination of DC vaccine with low dose 5-FU chemotherapy induced complete tumor regression in 75% of the treated animals.

Conclusion

Taken together our results indicate that low dose 5-FU plus DC vaccine can enhance the antitumor response and lead to complete tumor regression.

Finacial support

FAPESP 2009/18331-8; 2010/06013-9.

Copyright information

© de Camargo et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Marcela R de Camargo
    • 1
    • 2
  • Carolina M Gorgulho
    • 1
  • Cecília P Rodrigues
    • 1
  • Juliana CL Frederico
    • 1
    • 2
  • Fabiana A Zambuzi
    • 1
  • Victoria E Galvão
    • 1
  • Marcimara Penitenti
    • 3
  • Ramon Kaneno
    • 1
    • 2
  1. 1.Department of Microbiology and ImmunologyInstitute of Biosciences, UNESPBotucatuBrazil
  2. 2.Department of PathologySchool of Medicine, UNESPBotucatuBrazil
  3. 3.Fundação Amaral CarvalhoJaúBrazil

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