The prevalence of environmentral colonization of Legionella in hospital water systems in Taiwan – a 20 hospital surveillance
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KeywordsPneumonia Water Sample Drinking Water Standardize Protocol Healthcare Professional
Introduction / objectives
Legionnaires’ disease is a major cause of hospital and community acquired pneumonia. Hospital-acquired Legionnaires’ disease is directly linked to the presence of Legionella in hospital drinking water. The objective is to systematically investigate the presence of Legionella and its colonization rate in hospital water systems in Taiwan.
Twenty hospitals (Hospitals A to T) throughout Taiwan (8 in northern, 2 in central, 7 in southern, 2 in eastern Taiwan, and one in rural island) were cultured for Legionella. We followed the standardized protocol to perform environmental cultures using (1) water samples; (2) BCYE and DGVP culture media; (3) latex agglutination test (LAX) and direct fluorescent antibody (DFA) technique for L. pneumophila speciation and serotyping. We also perform speciation for L. micdadei since it is implicated in transplant patients.
Among 706 water samples collected during 2009 ~ 2011 period, 21% (149/706) were positive for Legionella. 65% (13/20) of hospital water systems are positive for Legionella; 2 have >30% site positive, 7 are between 10% ~ 30% site positive, and 4 are <10% site positive. L. pneumophila serogroups 1, 3, 5, 6, 7, were isolated from 62% (8/13), 31% (4/13), 8% (1/13), 38% (5/13), and 8% (1/13) of the hospitals, respectively. Five hospitals yielded L. species, but none of them were L. micdadei.
This study allow health official and healthcare professionals for the development of water safety plan to better protect patients and residents of Taiwan in an attempt to prevent Legionnaires’ diseases.
Disclosure of interest
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.