Leukemoid reaction and autocrine growth of bladder cancer induced by paraneoplastic production of granulocyte colony-stimulating factor – a potential neoplastic marker: a case report and review of the literature
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Granulocyte colony-stimulating factor produced by nonhematopoietic malignant cells is able to induce a leukemoid reaction by excessive stimulation of leukocyte production. Expression of granulocyte colony-stimulating factor and its functional receptors have been confirmed in bladder cancer cells. In vitro studies have demonstrated that granulocyte colony-stimulating factor/receptor exhibits a high affinity binding and this biological axis increases proliferation of the carcinoma. Urothelial carcinoma of the bladder is rarely associated with a leukemoid reaction and autocrine growth induced by paraneoplastic production of granulocyte colony-stimulating factor. In the world literature, there have been less than 35 cases reported in the last 35 years. The clinicopathological aspects, biology, prognosis and management of granulocyte colony-stimulating factor-secreting bladder cancers are poorly understood.
A 39-year-old Caucasian woman with an invasive high-grade urothelial carcinoma presented with hematuria and low-grade fevers. Laboratory tests revealed an elevated white blood cell count and absolute neutrophil count and an elevated 24-hour urine protein. Upon further evaluation she was found to have locally advanced high-grade urothelial carcinoma without nodal or distant metastasis. Her serum granulocyte colony-stimulating factor level was 10 times the normal limit. This led to the diagnosis of a paraneoplastic leukemoid reaction. Her white blood cell count immediately normalized after cystectomy but increased in concordance with recurrence of her disease. Unfortunately, she rapidly progressed and expired within 10 months from the time of first diagnosis.
This is one of the few cases reported that illustrates the existence of a distinct and highly aggressive subtype of bladder cancer which secretes granulocyte colony-stimulating factor. Patients presenting with a leukemoid reaction should be tested for granulocyte colony-stimulating factor/receptor biological axis. Moreover, granulocyte colony-stimulating factor could be a potential neoplastic marker as it can follow the clinical course of the underlying tumor and thus be useful for monitoring its evolution. Neoadjuvant chemotherapy should be considered in these patients due to the aggressive nature of these tumors. With a better understanding of the biology, this autocrine growth signal could be a potential target for therapy in future.
KeywordsBladder cancer Granulocyte colony-stimulating factor Leukemoid reaction
Absolute neutrophil count
Erythrocyte sedimentation rate
Granulocyte colony-stimulating factor
Granulocyte colony-stimulating factor receptor
white blood cell.
Paraneoplastic leukemoid reaction is a type of paraneoplastic syndrome in which there is an elevation in the white blood cell count, predominantly neutrophils. Granulocyte colony-stimulating factor (G-CSF) secreted by nonhematopoietic malignant cells has been reported to be able to induce a leukemoid reaction through excessive stimulation of leukocyte production [1, 2, 3]. Urothelial carcinoma of the bladder is rarely associated with a leukemoid reaction, with less than 30 cases reported over the past 30 years, some of which tried to elucidate the role of G-CSF [1, 3, 4, 5, 6, 7]. A previous study reported that 9.2% of bladder tumors are associated with high levels of G-CSF . In addition, expression of G-CSF and functional G-CSF receptors (G-CSFR) has been demonstrated in bladder cancer cells . In vitro studies have demonstrated that G-CSF/G-CSFR exhibit high affinity  binding and this biological axis increases proliferation in bladder cancer cells [1, 9, 10, 11]. This autocrine mechanism of growth may be associated with aggressive tumor growth and adverse clinical outcomes [1, 12]. Here, we present a rare case of a leukemoid reaction and autocrine growth of bladder cancer induced by paraneoplastic production of G-CSF. We review the literature on the main clinicopathological aspects of this important, but rare, condition and review the biology of G-CSF in bladder cancer and its implications for diagnosis, management, prognosis and future research.
Comparison of laboratory values before and after cystectomy
Total white blood cell (K/UL)
Absolute neutrophil count (K/UL)
Leukocyte alkaline phosphatase score
Erythrocyte sedimentation rate (mm/hour)
C-reactive protein (mg/dL)
24-hour urine protein (g)
Serum creatinine (mg/dL)
The patient refused adjuvant chemotherapy and returned 3 months following her cystectomy with an elevated WBC (peak WBC 45.3K/UL) and serum creatinine 1.90. A CT scan showed multiple pelvic masses with abdominal lymphadenopathy and a large right pelvic mass in close approximation to the area of the sigmoid colon causing obstruction. Her serum G-CSF level was 406.6pg/mL (normal <39.1pg/mL) confirming the diagnosis of paraneoplastic leukemoid reaction. She underwent a diverting colostomy to relieve symptoms of bowel obstruction. However due to poor performance status, renal failure and the development of a colovaginal fistula, she was placed on palliative care and expired soon thereafter.
This rare case of urothelial carcinoma of the bladder had several unusual features including young age at presentation, marked leukemoid reaction, elevated G-CSF levels (ten times the normal limit), nephrotic range proteinuria and very rapid progression of disease to death within 6 months of the onset of the leukemoid reaction. Urothelial carcinoma in this case showed squamous differentiation which has been reported to have elevated G-CSF levels [4, 5, 6]. A possible mechanism for development of leukemoid reaction and rapid metastatic spread of the tumor is that the high-grade urothelial carcinoma harbored both paraneoplastic production of G-CSF and expression of functional G-CSFR. This may have stimulated the bone marrow with increased myelopoiesis and leukemoid reaction. The stimulation of tumor growth may have been due to the expression of functional G-CSFR by the neoplastic cells, creating a positive feedback cycle. This mechanism has been demonstrated in in vitro studies [1, 9, 11]. Consistent with this mechanism, the above unusual features normalized after removal of the tumor (Table 1 and Figure 1). Similar post-surgical normalization of the leukemoid reaction and G-CSF has been previously observed in bladder cancer [5, 13]. Three months later, with recurrence of tumor, there was elevated WBC and G-CSF. Hence, G-CSF could be used to follow the evolution of cancer and as a potential future prognostic marker. Of note, this case also exhibited nephrotic range proteinuria which improved after tumor removal, thereby clinically seemed to be paraneoplastic, but biopsy showed features of diabetic nephropathy. Minimal-change glomerulopathy associated with urothelial carcinoma has been rarely reported in the literature [14, 15]. However, this may have been masked by her pre-existing diabetic nephropathy.
In the present case, as the tumor recurred and grew in her pelvic cavity, the concentration of serum G-CSF was elevated with an associated increase in her WBC count. These findings support the hypothesis that the bladder cancer cells producing G-CSF may grow more rapidly or that the G-CSF production exhibited by the tumor cells may promote the malignant progression. We thus undertook a comprehensive review of all studies and similar cases reported to date to explore the above hypothesis. It is also probable that the patient had micrometastatic disease, undetectable by currently existing diagnostic modalities, prior to surgery. Rapid development of metastases within 3 months after surgery may have been invigorated by G-CSF secretion and activation of the G-CSF/G-CSFR biological axis stimulating tumor growth.
A review by Turalic et al. showed that the average age of these patients was 70.1 years and the male to female ratio was 3:2. The WBC was as high as 181,800/mL (average 66,758/mL) and there was an average G-CSF level of 203pg/mL with a mean survival of 5.9 months following surgical therapy in these patients. However, those who received adjuvant chemotherapy reported survivals of >20 months . An observational study in Japanese patients by Mizutani et al. in 1995 showed that 9.2% of bladder tumors had elevated G-CSF levels (mean G-CSF of 328), which positively correlated with an increase in grade and progression of stage of cancer, more so in patients with distant metastasis. Furthermore, patients with bladder cancer without elevated G-CSF had better disease-specific survival rates at 5 years follow-up when compared with those with an elevated level of serum G-CSF .
The mechanism responsible for overexpression of G-CSF in bladder cancer is yet to be clearly elucidated. Possibilities include rearrangement of the G-CSF gene occurring within one of the alleles  and intrinsic activation of nuclear factors that work on the promoter region of the G-CSF gene . Treatment with phorbol ester, interleukin-1 beta, or interferon gamma increased the level of G-CSF, granulocyte-macrophage colony-stimulating factor, and macrophage colony-stimulating factor in an in vitro bladder cancer cell line study. This capability to respond to various stimuli implies that numerous regulatory pathways may be involved in the production of cytokines . Although the mechanism of G-CSF gene overexpression requires further investigation, Chakraborty et al. conducted a series of in vitro studies which have shown that G-CSFR specifically signals for beta-1 integrin expression, adhesion and invasion of bladder cancer cells which could promote metastasis . Furthermore, they demonstrated that the G-CSF/G-CSFR biological axis facilitated survival and growth of bladder cancer cells and stimulated STAT3-dependent survivin expression . The histogenesis of transitional cell carcinoma of the bladder is uncertain, although different theories have been proposed. Some authors have suggested that a metaplastic phenomenon with various degrees of differentiation may be responsible for the malignant transitional cell G-CSF production . In addition, this theory is supported further by the remarkable potential of the transitional epithelium and transitional cell carcinoma to differentiate into several lines . The frequent finding of both squamous and glandular differentiation has long been observed in transitional cell carcinoma. Furthermore, the presence of neuroendocrine (small cell) differentiation has also been reported . Hematopoietic differentiation of transitional cell carcinoma, resulting in the acquirement of G-CSF production and G-CSFR expression, is another possibility supported by our observations. Lastly, G-CSF may be released from the foci of tumor necrosis . Hence, G-CSF could be a potential screening tool to predict aggressive cancer and hence pursue an early aggressive treatment modality.
G-CSF administration has been in vogue as an adjunct to the management of granulocytopenias, either with or without prior chemotherapy [23, 24, 25, 26]. Subsequent studies have further confirmed the efficacy of G-CSF in conjunction with systemic chemotherapy for patients with advanced bladder cancer [27, 28]. A few studies recommend G-CSF administration to enhance the tumor-specific cytotoxicity of chemotherapy [29, 30], to allow dose intensification of chemotherapy [31, 32, 33] with low myelotoxicity and mucosal toxicity levels . In contrast, Perez et al. reported a case of rapid clinical deterioration and leukemoid reaction after treatment of urothelial carcinoma with G-CSF along with chemotherapy . Recombinant G-CSF may have both direct and indirect stimulatory effects on the growth of bladder cancer cells in vitro. It may stimulate the growth of the residual tumor cells after chemotherapy while it is being used clinically to combat myelosuppression induced by antitumor chemotherapy in bladder cancer . Due to controversial reports, at this time, we can only advocate that all bladder cancers should be tested for the expression of G-CSFR before using G-CSF, because there is the possibility of growth stimulation by G-CSF if they have G-CSFR on their cell surfaces. With a better understanding of the biology, this autocrine growth signal could be a potential target for therapy in future.
On the basis of this case experience and review of literature we conclude that there is a distinct and highly aggressive subtype of bladder cancer which secretes G-CSF and expresses G-CSFR inducing an autocrine growth cycle. Hence such patients presenting with leukemoid reaction should be tested for G-CSF/G-CSFR biological axis. Moreover, G-CSF could be a potential neoplastic marker as it can follow the clinical course of the underlying tumor and thus be useful for monitoring its evolution. Neoadjuvant chemotherapy should be considered as the aggressive nature of these tumors appears to overwhelm these patients before they receive adjuvant treatments. With a better understanding of the biology, this autocrine growth signal could be a potential target for therapy in future.
We are currently planning to conduct a preliminary study to identify patients with such bladder cancer, not only to estimate its prevalence but also to assess the variation in G-CSF levels in response to various therapies (surgery, chemotherapy, radiotherapy). In addition we will evaluate G-CSF as a prognostic marker and as a tool to predict a more aggressive biological phenotype of invasive bladder cancer. Future studies will be necessary to determine the regulatory effects of G-CSF on bladder cancer cells.
Written informed consent was obtained from the patient’s next of kin for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
The authors would like to thank Dr Ivan Damjanov, Pathologist at University of Kansas Medical Center, Kansas for providing biopsy descriptions and picture (Figure 4).
- 15.Cases A, Torras A, Lens XM, Revert L: Minimal-change nephrotic syndrome associated with an undifferentiated urothelial carcinoma. Med Clin (Barc). 1986, 86: 436-Google Scholar
- 16.George DD, Griffin JD: Granulocyte colony stimulating factor and its receptor. Blood 1991, 78:2791–2795 (cited by Mizutani Y, Okada Y, Terachi T, Kakehi Y, Yoshida O: Serum granulocyte colony-stimulating factor levels in patients with urinary bladder tumour and various urological malignancies. Br J Urol. 1995, 76: 580-586. 10.1111/j.1464-410X.1995.tb07782.x.CrossRefGoogle Scholar
- 18.Steube KG, Meyer C, Tachibana M, Murai M, Drexler HG: Bladder carcinoma cell line KU-19-19-derived cytokines support proliferation of growth factor-dependent hematopoietic cell lines: modulation by phorbol ester, interferon-gamma and interleukin-1 beta. Biochem Biophys Res Commun. 1998, 242: 497-501. 10.1006/bbrc.1997.8002.CrossRefPubMedGoogle Scholar
- 21.Grammatico D, Grignon DJ, Eberwein P, Shepherd RR, Hearn SA, Walton JC: Transitional cell carcinoma of the renal pelvis with choriocarcinomatous differentiation. Immunohistochemical and immunoelectron microscopic assessment of human chorionic gonadotropin production by transitional cell carcinoma of the urinary bladder. Cancer. 1993, 71: 1835-1841. 10.1002/1097-0142(19930301)71:5<1835::AID-CNCR2820710519>3.0.CO;2-5.CrossRefPubMedGoogle Scholar
- 23.Gabrilove JL, Jakubowski A, Scher H, Sternberg C, Wong G, Grous J, Yagoda A, Fain K, Moore MA, Clarkson B, Oettgen HF, Alton K, Welte K, Souza L: Effect of granulocyte colony-stimulating factor on neutropenia and associated morbidity due to chemotherapy for transitional-cell carcinoma of the urothelium. N Engl J Med. 1988, 318: 1414-1422. 10.1056/NEJM198806023182202.CrossRefPubMedGoogle Scholar
- 24.Crawford J, Ozer H, Stoller R, Johnson D, Lyman G, Tabbara I, Kris M, Grous J, Picozzi V, Rausch G, Smith R, Gradishar W, Yahanda A, Vincent M, Stewart M, Glaspy J: Reduction by granulocyte colony-stimulating factor of fever and neutropenia induced by chemotherapy in patients with small-cell lung cancer. N Engl J Med. 1991, 325: 164-170. 10.1056/NEJM199107183250305.CrossRefPubMedGoogle Scholar
- 26.Ohno R, Tomonaga M, Kobayashi T, Kanamaru A, Shirakawa S, Masaoka T, Omine M, Oh H, Nomura T, Sakai Y, Hirano M, Yokomaku S, Nakayama S, Yoshida Y, Miura AB, Morishima Y, Dohy H, Niho Y, Hamajima N, Takaku F: Effect of granulocyte colony-stimulating factor after intensive induction therapy in relapsed or refractory acute leukemia. N Engl J Med. 1990, 323: 871-877. 10.1056/NEJM199009273231304.CrossRefPubMedGoogle Scholar
- 30.Akaza H, Fukushima H, Koiso K, Aso Y: Enhancement of chemotherapeutic effects by recombinant human granulocyte colony-stimulating factor on implanted mouse bladder cancer cells (MBT-2). Cancer. 1992, 69: 997-1002. 10.1002/1097-0142(19920215)69:4<997::AID-CNCR2820690428>3.0.CO;2-U.CrossRefPubMedGoogle Scholar
- 31.Viens P, Gravis G, Bladou F, Lechevallier E, Baume D, Camerlo J, Cowen D, Coulange C, Serment G, Resbeut M, Maraninchi D: Impact of recombinant human granulocyte colony stimulating factor on dose intensity and toxicity of three cycles of methotrexate, vinblastine, doxorubicin and cisplatin in patients with previously untreated urothelial bladder carcinoma. Eur Cytokine Netw. 1996, 7: 395-399.PubMedGoogle Scholar
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